Methods for purifying biological cells

1. A method for purifying viable cells, comprised of:

a) providing at least one column, wherein the column is comprised of a packed bed of medium, wherein the packed bed of medium has a bed volume, wherein the packed bed of medium is retained between two frits, a lower frit and an upper frit, wherein the packed bed of medium is comprised of particles, wherein the particles are comprised of an affinity group, wherein the affinity group is an aptamer;

b) providing a biological sample comprised of viable cells, wherein the biological sample has a sample volume, wherein the sample volume is larger than the bed volume;

c) aspirating the biological sample through the column using bidirectional flow, wherein the sample is aspirated through the lower frit, into the packed bed of medium and then through the upper frit;

d) expelling the biological sample, wherein the biological sample is expelled through the upper frit, into the packed bed of medium and then through the lower frit;

e) repeating steps (c) and (d) multiple times, wherein the biological sample is not incubated on the column, whereby a portion of the viable cells are captured by the aptamer affinity groups on the particles in the packed bed of medium;

f) aspirating a wash solution through the column using bidirectional flow, wherein the wash solution is aspirated through the lower frit, into the packed bed of medium and then through the upper frit;

g) expelling the wash solution through the upper frit, into the packed bed of medium and then through the lower frit; and

h) eluting the cells by passing an eluent through the column, wherein the eluted cells are viable, wherein the method is at least partially performed by a timed computer-controlled program, and wherein the entire method is performed in less than one hour.

2. The method of claim 1 , wherein between steps (g) and (h), the captured cells are labeled with a dye on the column.

3. The method of claim 1 , wherein the column is a pipette tip.

4. The method of claim 1 , wherein the sample is comprised of biological fluids or tissue.

5. The method of claim 1 , wherein the viable cells in the biological sample are T cells.

6. The method of claim 1 , wherein the frits have a pore size, and wherein the pore size is small enough to contain the packed bed of medium but large enough for cells to pass through.

7. A method purifying viable cells, comprised of:

a) providing a at least one column, wherein the column is comprised of a packed bed of medium, wherein the packed bed of medium has a bed volume, wherein the packed bed of medium is retained between two frits, a lower frit and an upper frit, wherein the packed bed of medium is comprised of particles, wherein the particles are comprised of an affinity group, wherein the affinity group is an aptamer;

b) providing a biological sample comprised of viable cells, wherein the viable cells are not labeled with antibodies, wherein the biological sample has a sample volume, wherein the sample volume is larger than the bed volume;

c) aspirating the biological sample through the column using bidirectional flow, wherein the sample is aspirated through the lower frit, into the packed bed of medium and then through the upper frit;

d) expelling the biological sample, wherein the biological sample is expelled through the upper frit, into the packed bed of medium and then through the lower frit;

e) repeating steps (c) and (d) multiple times, wherein the biological sample is not incubated on the column, whereby a portion of the viable cells are captured on aptamer affinity groups on the particles in the packed bed of medium;

f) aspirating a wash solution through the column using bidirectional flow, wherein the wash solution is aspirated through the lower frit, into the packed bed of medium and then through the upper frit;

g) expelling the wash solution through the upper frit, into the packed bed of medium and then through the lower frit; and

h) eluting the cells by passing an eluent through the column, wherein the eluted cells are viable and wherein the method is at least partially performed by a timed computer-controlled program.

8. The method of claim 7 , wherein between steps (g) and (h), the captured cells are labeled with a dye on the column.

9. The method of claim 7 , wherein the column is a pipette tip.

10. The method of claim 7 , wherein cells in the sample is comprised of biological fluids or tissue.

11. The method of claim 7 , wherein the cells in the biological sample are T cells or sperm cells.