Leucine-rich peptide compositions and methods for isolation
Disclosed are compositions comprising isolated peptides having a leucine content of from about 12 to about 40 weight percent. Also disclosed is a method for isolating leucine-rich peptides from protein sources such as bovine whey and methods of use for these peptides to provide beneficial effects in a human and/or animal such as increasing blood flow, decreasing blood pressure, increasing muscle mass, improving cognitive function, improving cardiovascular function, etc.
1. A method for isolating leucine-rich peptides from a leucine-rich protein source, the method comprising the steps of:
a) hydrolyzing the protein in the presence of at least one leucyl aminopeptidase without additional proteases that are not leucyl aminopeptidases to form a protein hydrolysate,
b) deactivating the aminopeptidase(s), and
c) filtering the protein hydrolysate to produce a permeate containing leucine-rich peptides having a leucine content of from 12 to 40 weight percent and a molecular weight of from about 200 to about 4,000 in the permeate.
2. The method of claim 1 wherein the protein source is chosen from among the group consisting of legumes, fish, meat, milk, blood, egg, corn, wheat gluten, maize, and combinations thereof.
3. The method of claim 1 wherein the step of filtering is performed to collect peptides having a molecular weight of from about 200 to about 1,000.
4. The method of claim 1 wherein the step of filtering is performed to collect peptides having a molecular weight of from about 400 to about 1,000.
5. The method of claim 1 wherein the protein source is chosen from among the group consisting of bovine milk, bovine whey, whey protein concentrates, whey protein isolates, and combinations thereof.