Methods for the selective detection of alkyne-presenting molecules and related compositions and systems
View Patent ↗Provided herein are methods for selectively detecting an alkyne-presenting molecule in a sample and related detection reagents, compositions, methods and systems.
1. A method for selectively detecting an alkyne-presenting molecule in an untreated sample, the method comprising:
contacting the untreated sample with a detection reagent comprising an organic or organometallic label moiety presenting an azide or sulfonyl azide group;
the contacting performed for a time and under a condition for click chemistry to allow binding of the azide or sulfonyl azide group with one or more alkyne-presenting molecules possibly present in the untreated sample;
the contacting further performed in absence of a treatment of the untreated sample that introduces the one or more alkyne-presenting molecules into the untreated sample prior to the contacting;
wherein the binding of the azide or sulfonyl azide group to the alkyne-presenting molecules results in emission of a signal from the organic or organometallic label moiety; and
detecting the signal from the organic or organometallic label moiety.
2. The method of claim 1 , wherein the sample is an aqueous or organic solution.
3. The method of claim 1 , wherein the organic or organometallic label moiety comprises a positively charged moiety.
4. The method of claim 1 , wherein the organic or organometallic label moiety comprises a ferrocenyl moiety.
5. The method of claim 1 , wherein the detection reagent is selected from the group consisting of formulas XV-XVIII:
wherein:
R 1 and R 2 are independently C 1 -C 8 alkyl.
6. The method of claim 1 , wherein the detection reagent has a structure according to formula XIX:
wherein R is C 1 -C 8 alkyl, trifluoromethyl, or substituted or unsubstituted aryl.
7. The method of claim 1 , wherein the detection reagent is selected from the group consisting of Formulas XX-XIV:
8. The method of claim 1 , wherein the detecting is achieved by detecting a positive charge on the detection reagent in a mass spectrometer after the contacting.
9. The method of claim 1 , wherein the untreated sample is selected from a solid and/or fluid from a biological environment, specimen, cultures, tissues, or portions thereof.
10. The method of claim 1 , wherein the detecting the signal from the organic or organometallic label moiety comprises mass spectrometry.