3-dimensional large capacity cell encapsulation device assembly
Disclosed herein are implantable 3-dimensional large capacity device assemblies, specifically, large capacity device assemblies for encapsulating pancreatic progenitor cells for treatment of diabetes.
1. A cell encapsulating assembly, said assembly comprising at least two cell chambers and a cell-free region along the longest axis separating the cell chambers, wherein the cell-free region is bent 5 to 175 degrees.
2. The assembly of claim 1 , wherein the assembly comprises a semi-permeable membrane.
3. The assembly of claim 1 , wherein the assembly comprises at least three, four, five, six, seven, eight or more cell chambers.
4. The assembly of claim 1 , further comprising at least one loading port.
5. The assembly of claim 1 , further comprising definitive endoderm-lineage cells.
6. The assembly of claim 1 , further comprising human immature beta cells.
7. The assembly of claim 1 , further comprising a cell chamber matrix having a plurality of interconnected cavities or pores to disperse the living cells and to improve oxygen distribution inside the cell chamber.
8. The assembly of claim 7 , wherein the interconnected cavities have different cavity dimensions.
9. The assembly of claim 7 , wherein the matrix is polydimethylsiloxane (PDMS), polydimethylsiloxane mono acrylate, and polydimethylsiloxane monomethacrylate.
10. The assembly of claim 7 , wherein the matrix is a silicone elastomer.
11. The assembly of claim 7 , wherein the matrix is a polydimethylsiloxane (PDMS).
12. The assembly of claim 1 , wherein the cell chambers are separated by about 20 degrees.
13. The assembly of claim 1 , wherein the cell chambers are separated by about 40 degrees.
14. A encapsulating assembly, said assembly comprising at least two cell chambers, a cell-free region along the longest axis separating the cell chambers, wherein the cell-free region is bent 5 to 175 degrees, further comprising human endocrine precursor cells.
15. The assembly of claim 14 , further comprising human pancreatic and duodenal homeobox gene 1 (PDX1)-positive pancreatic progenitor cells.
16. The assembly of claim 1 , further comprising a partition seal within the cell chamber.
17. A cell encapsulating assembly, said assembly comprising at least two cell chambers and a cell-free region along the longest axis separating the cell chambers, wherein the cell-free region is bent 5 to 175 degrees and further comprising pancreatic and duodenal homeobox gene 1 (PDX1)-positive pancreatic progenitor cells.