IP Library › Granted Patent US 10,226,064
Granted Patent B2
US 10,226,064 · App. 14/206,666 · Granted Mar 12, 2019

Nitrite-degrading and TSNA-degrading bacteria and methods of making and using

Inventors: Ujwala Warek (Chester, VA); Mark T. Nielsen (Nicholasville, KY); Dongmei Xu (Glen Allen, VA); Mingwu Cui (Lexington, KY); Xue Luo (Richmond, VA); Xiaohong Jin (Glen Allen, VA); Elisabeth Miller (Chesterfield, VA); Kwang Choi (Glen Allen, VA)
Assignee: Altria Client Services LLC
A24B15/245A24B15/12A24B15/20C12N1/20
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Quick Facts
Patent No.
US 10,226,064
App. No.
14/206,666
Granted
Mar 12, 2019
Kind
B2
Abstract

Provided herein are bacterial strains that are capable of degrading nitrite and/or TSNAs. Also provided herein are methods of using such bacterial strains to degrade nitrite and/or TSNAs.

Claims (14)

1. A method of reducing the amount of nitrites in strong extract liquor (SEL), comprising contacting SEL with the isolated bacterial strain Myroides odoratus M20 (ATCC® Patent Deposit Designation PTA-125147) under conditions in which the amount of nitrite is reduced in the SEL, thereby producing a reduced-nitrite SEL, wherein the bacterial strain comprises the 16S rDNA sequence having the sequence shown in SEQ ID NO: 1.

2. The method of claim 1 , wherein the amount of nitrite in the SEL is reduced by at least 50%, compared to the amount of nitrite in the SEL prior to the contacting step.

3. The method of claim 1 , wherein the amount of nitrite in the SEL is reduced by at least 75%, compared to the amount of nitrite in the SEL prior to the contacting step.

4. The method of claim 1 , wherein the SEL is contacted with the isolated bacterial strain at a temperature of 26° C. to 37° C.

5. The method of claim 1 , wherein the SEL is contacted with the isolated bacterial strain at a temperature of 37° C.

6. The method of claim 1 , wherein the SEL is contacted with the isolated bacterial strain at a pH of 7.

7. The method of claim 1 , wherein the SEL is contacted with the isolated bacterial strain for less than 2 hours.

8. The method of claim 1 , wherein the SEL is contacted with the isolated bacterial strain for less than 10 hours.

9. The method of claim 1 , wherein the SEL is contacted with the isolated bacterial strain for less than 24 hours.

10. The method of claim 1 , wherein the SEL is contacted a second time with the isolated bacterial strain.

11. The method of claim 1 , further comprising processing the SEL to produce concentrated extract liquor (CEL).

12. The method of claim 1 , wherein the amount of nitrite in the SEL is reduced by at least 75%, compared to the amount of nitrite in the SEL prior to the contacting step.

13. The method of claim 1 , wherein the amount of nitrite in the SEL is reduced by at least 95%, compared to the amount of nitrite in the SEL prior to the contacting step.

14. The method of claim 1 , wherein the SEL is contacted with the isolated bacterial strain for less than 1 hour.

Assignments (2)
MERGER AND CHANGE OF NAME Recorded Oct 5, 2016
From: ALTRIA CLIENT SERVICES INC.; ALTRIA CLIENT SERVICES LLC
To: ALTRIA CLIENT SERVICES LLC
Reel/Frame 039948/0352 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 16, 2016
From: WAREK, UJWALA; NIELSEN, MARK T.; XU, DONGMEI; LUO, XUE; JIN, XIAOHONG; MILLER, ELISABETH; CHOI, KWANG
To: ALTRIA CLIENT SERVICES INC.
Reel/Frame 039763/0104 →
Continuity (2)
Provisional Application 61794854 · Mar 15, 2013
Related Publication 20140261481A1 · Sep 18, 2014