Apparatus and method for suppression of background noise in microscopy imaging
An apparatus and method for imaging a section of a medium is disclosed. The apparatus or method receives and returns light from the section and from sites adjacent to the section. A microscope using this apparatus or method can be telecentric in pinhole space at the detection end of the system.
1. An apparatus for imaging a section of a medium which receives and returns light from the section and from sites adjacent to the section, the apparatus comprising:
an optical system for directing light in beams of different polarization in the medium along an optical axis and returning light from the medium, the optical system comprising:
a pinhole lens assembly configured to receive the returned light, the pinhole lens assembly having a focal length, and wherein the focal length is configured such that a first focal point of the pinhole lens assembly is located substantially at a pupil of the optical system such that the pinhole assembly is telecentric; and
a detector configured to receive the returned light from the pinhole lens assembly and produce an electrical signal in response to the received light.
2. The apparatus of claim 1 , further comprising a processor in communication with the detector and configured to generate an image of the section based on the electrical signal of the detector.
3. The apparatus of claim 1 , wherein the electrical signal of the detector is configured to correspond to a polarization parameter of the received light.
4. The apparatus of claim 3 , wherein the polarization parameter is a function of a differential circular dichroism or optical activity of the returned light.
5. The apparatus of claim 3 , wherein the polarization parameter is a degree of rotation of the polarization of the returned light.
6. The apparatus of claim 1 , wherein the beams overlap in the medium to reduce a portion of the light returned from the sites adjacent to the section that are spaced generally along the optical axis.
7. The apparatus of claim 1 , wherein the beams are incident in the medium at spots spaced from each other along the optical axis.
8. A method of imaging a section of a medium which receives and returns light from the section and from sites adjacent to the section, the method comprising the steps of:
directing light in beams of different polarization to the medium along an optical axis;
directing light returned from the medium to a detector by way of a pinhole assembly;
causing the returned light to be substantially telecentric at the detector;
detecting, at the detector, the returned light; and
generating an image of the section from the detected light, the image generated in response to a polarization parameter of the returned light.
9. The method of claim 8 , wherein the beams overlap in the medium to reduce a portion of the light returned from the sites adjacent to the section which are spaced generally along the optical axis.
10. The method of claim 8 , wherein the beams are incident in the medium at spots spaced from each other along the optical axis.
11. The method of claim 8 , wherein the polarization parameter is a degree of rotation of the polarization of the returned light.
12. The method of claim 8 , wherein said polarization parameter is a function of a differential circular dichroism or optical activity of the returned light.