Bioactive compositions for high avidity cell capture
A composition useful for cell capture, the composition comprising a solid substrate on which is affixed a patterned polymer, and a cell-targeting agent attached to said patterned polymer, wherein said cell-targeting agent is exposed. Also described is a method for the preparation of the cell capturing composition, as well as flow through devices in which the cell capturing composition is incorporated. Further described is a method of capturing cells by contacting the cell-capturing composition with a liquid or gaseous sample containing cells. The method for capturing cells may also be a method for testing for the presence of one or more classes or species of cells or cellular organisms in a liquid or gaseous sample.
1. A composition useful for cell capture, the composition comprising a solid substrate on which is covalently affixed a patterned polymer having a substantially planar surface defined by x- and y-axes perpendicular to each other, and an array of regularly spaced protruded shapes on said substantially planar surface, wherein said array of regularly spaced protruded shapes has a set frequency of occurrence and a pattern density, each protruded shape being vertically raised along a z-axis perpendicular to the x- and y-axes of the substantially planar surface; having a height along the z-axis of at least 100 nm; and possessing an outer perimeter along the x- and y-axes, wherein said outer perimeter along the x- and y-axes for each protruded shape is vertically raised along the z-axis by a greater extent than portions of the z-axis of the protruded shape within the confines of the outer perimeter along the x- and y-axes, and cell-targeting agents covalently attached to said patterned polymer by a crosslinking group, wherein said cell-targeting agents are lectin and are exposed.
2. The composition of claim 1 , wherein said cell-targeting agent is a microbe-targeting agent.
3. The composition of claim 1 , wherein said patterned polymer is a block-copolymer.
4. The composition of claim 1 , wherein said patterned polymer is a vinyl addition polymer.
5. The composition of claim 1 , wherein said solid substrate is selected from the group consisting of metal, metal oxide, semiconductor, and plastic.
6. A method of capturing cells, the method comprising contacting a cell capturing composition with said cells, the cell-capturing composition comprising the composition of claim 1 .
7. The method of claim 6 , wherein said cell-targeting agent is a microbe-targeting agent.
8. The method of claim 6 , wherein said patterned polymer is a block-copolymer.
9. The method of claim 6 , wherein said patterned polymer is a vinyl addition polymer.
10. The method of claim 6 , wherein said solid substrate is selected from the group consisting of metal, metal oxide, semiconductor, and plastic.
11. The method of claim 6 , wherein said cells are microbes.
12. The method of claim 6 , wherein said cell-targeting agent is selective for a cell type.
13. The method of claim 6 , wherein said cell-targeting agent is selective for a genus or species of a microbe.
14. The method of claim 6 , wherein said cell-capturing composition is incorporated in a flow-through device.
15. The method of claim 6 , wherein said cells are within a liquid.
16. The method of claim 6 , wherein said cells are in air.
17. The composition of claim 1 , wherein said crosslinking group is a ring-opened azlactone group.
18. The composition of claim 1 , wherein each protruded shape has a height of at least 500 nm.
19. The composition of claim 1 , wherein said protruded shapes are selected from the group consisting of linear, circular, and polygonal protruded shapes.