Bispecific anti-EGFR/anti IGF-1R antibodies
The present invention relates to bispecific anti-EGFR/anti IGF-1R antibodies, methods for their production, pharmaceutical compositions containing said antibodies, and uses thereof.
1. A bispecific antibody that binds to human EGFR and human IGF-1R comprising a first antigen-binding site that binds to human EGFR and a second antigen-binding site that binds to human IGF-1R,
i) the first antigen-binding site comprises in the heavy chain variable domain a CDR1 of SEQ ID NO:1, a CDR2 of SEQ ID NO:2, and a CDR3 of SEQ ID NO:3, and in the light chain variable domain a CDR1 of SEQ ID NO:4, a CDR2 of SEQ ID NO:5, and a CDR3 of SEQ ID NO:6; and
ii) the second antigen-binding site is a modified antigen-binding site based on the heavy chain variable domain comprising a CDR1 of SEQ ID NO:9, a CDR2 of SEQ ID NO:10, and a CDR3 of SEQ ID NO:11; and
on the light chain variable domain comprising a CDR1 of SEQ ID NO: 12, a CDR2 of SEQ ID NO:13, and a CDR3 of SEQ ID NO:14;
wherein the modified second antigen-binding site comprises one more modifications in one or more of the CDRs; and
wherein the modified second antigen binding site has an at least 10-fold increased KD value of the binding affinity for binding to human IGF-1R compared to the unmodified second antigen binding site.
2. A bispecific antibody that binds to human EGFR and human IGF-1R comprising a first antigen-binding site that binds to human EGFR and a second antigen-binding site that binds to human IGF-1R, wherein
i) the first antigen-binding site comprises in the heavy chain variable domain a CDR1 of SEQ ID NO: 1, a CDR2 of SEQ ID NO: 2, and a CDR3 of SEQ ID NO:3, and in the light chain variable domain a CDR1 of SEQ ID NO: 4, a CDR2 of SEQ ID NO:5, and a CDR3 of SEQ ID NO:6; and
ii) a) the second antigen-binding site comprises in the heavy chain variable domain a CDR1 of SEQ ID NO:17, a CDR2 of SEQ ID NO:18, and a CDR3 of SEQ ID NO:19, and in the light chain variable domain a CDR1 of SEQ ID NO:20, a CDR2 of SEQ ID NO:21, and a CDR3 of SEQ ID NO:22; or
b) the second antigen-binding site comprises in the heavy chain variable domain_a CDR1 of SEQ ID NO:25, a CDR2 of SEQ ID NO:26, and a CDR3 of SEQ ID NO:27, and in the light chain variable domain a CDR1 of SEQ ID NO:28, a CDR2 of SEQ ID NO:29, and a CDR3 of SEQ ID NO:30; or
c) the second antigen-binding site comprises in the heavy chain variable domain_a CDR1 of SEQ ID NO:33, a CDR2 of SEQ ID NO:34, and a CDR3 of SEQ ID NO:35, and in the light chain variable domain a CDR1 of SEQ ID NO:36, a CDR2 of SEQ ID NO:37, and a CDR3 of SEQ ID NO:38; or
d) the second antigen-binding site comprises in the a heavy chain variable domain a CDR1 of SEQ ID NO:41, a CDR2 of SEQ ID NO:42, and a CDR3 of SEQ ID NO:43, and in the light chain variable domain a CDR1 of SEQ ID NO:44, a CDR2 of SEQ ID NO:45, and a CDR3 of SEQ ID NO:46; or
e) the second antigen-binding site comprises in the heavy chain variable domain a CDR1 of SEQ ID NO:49, a CDR2 of SEQ ID NO:50, and a CDR3 of SEQ ID NO:51, and in the light chain variable domain a CDR1 of SEQ ID NO:52, a CDR2 of SEQ ID NO:53, and a CDR3 of SEQ ID NO:54.
3. The bispecific antibody according to claim 2 , wherein
i) the first antigen-binding site comprises a heavy chain variable domain VH of SEQ ID NO: 7, and a light chain variable domain VL of SEQ ID NO: 8; and
ii) a) the second antigen-binding site comprises a heavy chain variable domain VH of SEQ ID NO: 23, and a light chain variable domain VL of SEQ ID NO: 24; or
b) the second antigen-binding site comprises a heavy chain variable domain VH of SEQ ID NO: 31, and a light chain variable domain VL of SEQ ID NO: 32; or
c) the second antigen-binding site comprises a heavy chain variable domain VH of SEQ ID NO: 39, and a light chain variable domain VL of SEQ ID NO: 40; or
d) the second antigen-binding site comprises a heavy chain variable domain VH of SEQ ID NO: 47, and a light chain variable domain VL of SEQ ID NO: 48; or
e) the second antigen-binding site comprises a heavy chain variable domain VH of SEQ ID NO: 55, and a light chain variable domain VL of SEQ ID NO: 56.
4. The bispecific antibody according to claim 2 , wherein said antibody is bivalent, trivalent or tetravalent.
5. The bispecific antibody according to claim 2 , wherein said antibody is glycosylated with a sugar chain at Asn297 whereby the amount of fucose within said sugar chain is 65% or lower.
6. A pharmaceutical formulation comprising a bispecific antibody according to claim 2 .