Fluidic connectors and microfluidic systems
Fluidic connectors, methods, and devices for performing analyses (e.g., immunoassays) in microfluidic systems are provided. In some embodiments, a fluidic connector having a fluid path is used to connect two independent channels formed in a substrate so as to allow fluid communication between the two independent channels. One or both of the independent channels may be pre-filled with reagents (e.g., antibody solutions, washing buffers and amplification reagents), which can be used to perform the analysis. These reagents may be stored in the channels of the substrate for long periods amounts of time (e.g., 1 year) prior to use.
1. A method of storing reagents comprising:
disposing a first reagent in a first microfluidic channel of a microfluidic system, wherein the first microfluidic channel comprises at least one inlet and one outlet, and wherein the microfluidic system comprises a second microfluidic channel including at least one inlet and one outlet;
disposing a second reagent in the second microfluidic channel; and
sealing the at least one inlet and outlet of the first microfluidic channel so as to store the first reagent in the first microfluidic channel,
wherein the first and second microfluidic channels are not in fluid communication with one another, and wherein the first and second microfluidic channels are constructed and arranged to be in fluid communication with one another by a fluidic connector that can connect to an inlet or an outlet of the first microfluidic and to an inlet or an outlet of the second microfluidic channel.
2. A method as in claim 1 , wherein the microfluidic system comprises a reaction area in fluid communication with the second microfluidic channel prior to the sealing step.
3. A method as in claim 1 , wherein the first reagent is a liquid reagent.
4. A method as in claim 1 , wherein the second reagent is a substantially dry reagent.
5. A method as in claim 1 , comprising disposing a third reagent in the first microfluidic channel.
6. A method as in claim 5 , wherein the third reagent is a liquid reagent.
7. A method as in claim 6 , wherein the first and third reagents are separated by a fluid that is immiscible with said first and third reagents.
8. A method as in claim 7 , wherein the fluid immiscible with the first and third reagents is a gas.
9. A method as in claim 8 , wherein at least one of the first and third reagents is a rinse solution.
10. A method as in claim 1 , comprising sealing the inlet of the second microfluidic channel and the outlet of the second microfluidic channel.
11. A method as in claim 2 , wherein the second reagent is disposed in the reaction area of the second microfluidic channel, and wherein the second reagent is a substantially dry reagent.
12. A method as in claim 11 , wherein the second reagent comprises at least one of an antibody or an antigen.
13. A method as in claim 11 , wherein the second reagent is adsorbed to a surface of the second microfluidic channel.
14. A method as in claim 2 , wherein the reaction area comprises at least one meandering channel region.
15. A method as in claim 14 , wherein the reaction area comprises at least two meandering channel regions connected in series.
16. A method as in claim 15 , wherein each of the at least two meandering channel regions comprises a chemical and/or biological species disposed therein that can undergo a chemical and/or biological reaction.
17. A method as in claim 16 , wherein the chemical and/or biological reaction is a binding event between at least two binding partners, and wherein at least one of the binding partners comprises an antibody.
18. A method as in claim 2 , wherein the reaction area comprises at least one cross-sectional dimension of less than 100 microns.
19. A method as in claim 1 , wherein the microfluidic system comprises a substrate comprising the first and second microfluidic channels formed therein.
20. A method as in claim 19 , wherein the substrate comprises more than one substrate layers that are mated to one another.
21. A method as in claim 1 , comprising disposing the second reagent in the second microfluidic channel prior to disposing the first reagent in the first microfluidic channel.
22. A method as in claim 1 , wherein disposing the second reagent in the second microfluidic channel comprises placing a fluid containing the second reagent in the second microfluidic channel, removing at least a portion of the fluid from the second microfluidic channel, and enclosing at least a portion of the second microfluidic channel with a cover.
23. A method as in claim 22 , wherein the first reagent is a liquid reagent, the method comprising disposing a third, liquid reagent in the first microfluidic channel, wherein the first and third reagents are separated by a gas that is immiscible with the first and third reagents in the first microfluidic channel.
24. A method as in claim 23 , wherein the microfluidic system comprises a substrate comprising-more than one substrate layers that are mated to one another.