COMPOSITIONS AND METHODS FOR THE PRODUCTION OF ISOPRENE
The present disclosure describes compositions and methods for production of isoprene from lignocellulosic plant biomass using a genetically engineered strain of a saprophytic bacteria.
1 . A saprophytic bacteria comprising an isoprene synthase.
2 . The saprophytic bacteria of claim 1 , wherein the saprophytic bacteria is Saccharophagus degradans 2-40.
3 . The saprophytic bacteria of claim 1 , wherein the isoprene synthase is selected from the group consisting of quaking aspen isoprene synthase, kudzu isoprene synthase and peanut isoprene synthase.
4 . The saprophytic bacteria of claim 1 , wherein the saprophytic bacteria comprises a heterologous nucleic acid encoding isoprene synthase.
5 . The saprophytic bacteria of claim 4 , wherein the heterologous nucleic acid is operably linked to a promoter selected from a tac promoter and a cel9A promoter.
6 . The saprophytic bacteria of claim 4 , wherein the heterologous nucleic acid matches codon usage of Saccharophagus degradans 2-40.
7 . A method of producing isoprene comprising
(a) providing a saprophytic bacteria comprising an isoprene synthase; and
(b) culturing the saprophytic bacteria in a media comprising a carbon source, thereby producing isoprene.
8 . The method of claim 7 , wherein the saprophytic bacteria is Saccharophagus degradans 2-40.
9 . The method of claim 8 , wherein the growth media further comprises mineral salts from sea water.
10 . The method of claim 7 , wherein the growth media further comprises an inorganic nitrogen source.
11 . The method of claim 7 , wherein the carbon source is selected from the group consisting of glucose, sucrose, lactose, starch, cellulose, hemicellulose and corn cob.
12 . The method of claim 7 , wherein the isoprene synthase is selected from the group consisting of quaking aspen isoprene synthase, kudzu isoprene synthase and peanut isoprene synthase.
13 . The method of claim 7 , wherein the saprophytic bacteria comprises a heterologous nucleic acid encoding isoprene synthase.
14 . The method of claim 13 , wherein the heterologous nucleic acid is operably linked to a promoter selected from a tac promoter and a cel9A promoter.
15 . The method of claim 8 , wherein the heterologous nucleic acid matches codon usage of Saccharophagus degradans 2-40.
16 . A method of producing a bacteria that produces isoprene in the presence of a carbon source comprising
(a) providing a saprophytic bacteria; and
(b) introducing to the saprophytic bacteria a heterologous nucleic acid encoding an isoprene synthase,
thereby producing a bacteria that produces isoprene in the presence of a carbon source.
17 . The method of claim 16 , wherein the saprophytic bacteria is Saccharophagus degradans 2-40.
18 . The method of claim 16 , wherein the carbon source is selected from the group consisting of glucose, sucrose, lactose, starch, cellulose, hemicellulose and corn cob.
19 . The method of claim 16 , wherein the isoprene synthase is selected from the group consisting of quaking aspen isoprene synthase, kudzu isoprene synthase and peanut isoprene synthase.
20 . The method of claim 17 , wherein the heterologous nucleic acid is operably linked to a promoter selected from a tac promoter and a cel9A promoter.
21 . The method of claim 17 , wherein the heterologous nucleic acid matches codon usage of Saccharophagus degradans 2-40.