Genes and proteins for alkanoyl-CoA synthesis
View Patent ↗Polypeptides having alkanoyl-CoA activity have been identified and characterized, as have nucleic acids encoding these polypeptides. Expression or over-expression of the nucleic acids alters levels of cannabinoid compounds in organisms. The polypeptides may be used in vivo or in vitro to produce cannabinoid compounds.
1. A process of decreasing levels of a cannabinoid compound in a cannabis plant, cannabis cell or cannabis tissue, the process comprising using a RNAi nucleic acid molecule comprising a nucleotide sequence complementary to a portion of SEQ ID NO: 1 or SEQ ID NO: 3, to silence in the cannabis plant, cannabis cell or cannabis tissue a gene that encodes an enzyme that catalyzes synthesis of an alkanoyl-CoA, in comparison to a similar variety of organism, cell or tissue grown under similar conditions but without the use of the nucleic acid molecule for silencing.
2. The process of claim 1 , wherein the cannabinoid compound is one or more of cannabigerolic acid, Δ 9 -tetrahydrocannabinolic acid, cannabidiolic acid, cannabichromenic acid, Δ 9 -tetrahydrocannabinol, cannabidiol or cannabichromene or an analog thereof comprising a side-chain of 1 to 9 carbon atoms in length.
3. The process of claim 1 , wherein the nucleotide sequence is complementary to a portion of SEQ ID NO:1.
4. The process of claim 1 , wherein the nucleotide sequence is complementary to a portion of SEQ ID NO: 3.
5. The process of claim 2 , wherein the nucleotide sequence is complementary to a portion of SEQ ID NO: 1.
6. The process of claim 2 , wherein the nucleotide sequence is complementary to a portion of SEQ ID NO: 3.
7. The process of claim 1 , wherein the use of the nucleic acid molecule comprises a method selected from RNAi, amiRNA, VIGS virus, antisense oligonucleotide, targeted mutagenesis and Targeting Induced Local Lesions IN Genomes (TILLING).
8. The process of claim 1 , wherein the enzyme is hexanoyl-CoA synthetase.
9. The process of claim 2 , wherein the enzyme is hexanoyl-CoA synthetase.
10. The process of claim 1 , wherein the alkanoyl-CoA is hexanoyl-CoA.
11. The process of claim 1 , wherein the nucleotide sequence has a length of at least 300 base pairs.