Insect resistant and herbicide tolerant breeding stack of soybean event pDAB9582.814.19.1 and pDAB4468.04.16.1
A breeding stack of soybean event 9582.814.19.1 and soybean event pDAB4468.04.16.1 resulted in the novel soybean event pDAB9582.814.19.1::pDAB4468.04.16.1. Soybean event pDAB9582.814.19.1::pDAB4468.04.16.1 comprises genes encoding AAD-12, Cry1F, Cry1Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products.
1. A method of controlling insects, said method comprising contacting insects with insect resistant soybean plants of event pDAB9582.814.19.1::pDAB4468.04.16.1, wherein said event comprises SEQ ID NO: 14, encoding cry1F, cry1Ac and phosphinothricin acetyltransferase (pat), and SEQ ID NO: 15, encoding aryloxyalkanoate dioxygenase-12 (aad-12) and pat, to thereby control insects selected from the group consisting of Pseudoplusia includens, Anticarsia gemmatalis and Spodoptera frugiperda.
2. The method of claim 1 wherein said insects are Pseudoplusia includens.
3. The method of claim 1 wherein said insects are Anticarsia gemmatalis.
4. The method of claim 1 wherein said insects are Spodoptera frugiperda.
5. A method of controlling weeds in an area comprising a soybean crop, said method comprising applying a glufosinate herbicide, a phenoxyacetic acid herbicide, a pyridyloxyacetic acid herbicide, or combinations thereof to said, wherein soybean plants of said soybean crop comprise event pDAB9582.814.19.1::pDAB4468.04.16.1, wherein said event comprises SEQ ID NO: 14, encoding cry1F, cry1Ac and pat, and SEQ ID NO: 15, encoding aad-12 and pat.
6. An isolated DNA molecule comprising one and more sequences selected from the group consisting of nucleotides 1385-1415 of SEQ ID NO: 1; nucleotides 1350-1450 of SEQ ID NO: 1; nucleotides 1300-1500 of SEQ ID NO: 1; nucleotides 1200-1600 of SEQ ID NO: 1; nucleotides 137-168 of SEQ ID NO:2; bp 103-203 of SEQ ID NO:2; and bp 3-303 of SEQ ID NO:2; or one or more sequences selected from the group consisting of nucleotides 2680-2780 of SEQ ID NO: 15; nucleotides 2630-2830 of SEQ ID NO:15; nucleotides 2530-2930 of SEQ ID NO:15; nucleotides 9071-9171 of SEQ ID NO:15; nucleotides 9021-9221 of SEQ ID NO: 15; and nucleotides 8921-9321 of SEQ ID NO: 15.
7. A method of breeding a soybean plant, comprising:
crossing a first soybean plant with a second soybean plant to produce a third soybean plant, wherein said first plant is a plant of event pDAB9582.814.19.1, wherein said event comprises SEQ ID NO: 14, encoding cry1F, cry1Ac and pat, and wherein said second plant is a plant of event pDAB4468.04.16.1, wherein said event comprises SEQ ID NO: 15, encoding aad-12 and pat; and
assaying said third soybean plant for presence of DNA comprising said SEQ ID NO: 14 and SEQ ID NO: 15.
8. An isolated DNA molecule comprising a junction sequence comprising at least one sequence selected from the group consisting of nucleotides 1385-1415 of SEQ ID NO:1; nucleotides 1350-1450 of SEQ ID NO:1; nucleotides 1300-1500 of SEQ ID NO:1; nucleotides 1200-1600 of SEQ ID NO:1; nucleotides 137-168 of SEQ ID NO:2; nucleotides 103-203 of SEQ ID NO:2; nucleotides 3-303 of SEQ ID NO:2 and full complements thereof; and comprising at least one sequence selected from the group consisting of bp 2680-2780 of SEQ ID NO:15; nucleotides 2630-2830 of SEQ ID NO: 15; nucleotides 2530-2930 of SEQ ID NO:15; nucleotides 9071-9171 of SEQ ID NO:15; nucleotides 9021-9221 of SEQ ID NO:15; nucleotides 8921-9321 of SEQ ID NO:15 and full complements thereof.
9. A soybean plant that is resistant to Pseudoplusia includens , wherein said plant comprises event pDAB9582.814.19.1::pDAB4468.04.16.1, wherein said event comprises SEQ ID NO: 14, encoding cry1F, cry1Ac and pat, and SEQ ID NO: 15, encoding aad-12 and pat.
10. A seed of the plant of claim 9 , wherein said seed comprises said event.
11. Soybean meal produced from the plant of claim 9 , wherein said meal comprises said event.
12. A method of controlling insect pests selected from the group consisting of Pseudoplusia includens, Anticarsia gemmatalis , and Spodoptera frugiperda , in soybean seed, meal, or flour, said method comprising contacting said insects with seed, meal, or flour that comprises event pDAB9582.814.19.1::pDAB4468.04.16.1, wherein said event comprises SEQ ID NO: 14 encoding cry1F, cry1Ac and pat, and SEQ ID NO: 15, encoding aad-12 and pat.
13. A part of the soybean plant of claim 9 , wherein said part is selected from the group consisting of pollen, ovule, flowers, shoots, roots, and leaves, wherein said part comprises said event.