Patent

1. A recombinant Trichoderma host cell, comprising polynucleotides encoding: (i) an Aspergillus fumigatus cellobiohydrolase I; (ii) an Aspergillus fumigatus cellobiohydrolase II; (iii) an Aspergillus fumigatus beta-glucosidase; (iv) an Aspergillus fumigatus GH61 polypeptide having cellulolytic enhancing activity; (v) an Aspergillus fumigatus xylanase; and (vi) an Aspergillus fumigatus beta-xylosidase; or homologs thereof;

wherein the Aspergillus fumigatus cellobiohydrolase I or homolog thereof is selected from the group consisting of:

(i) a cellobiohydrolase I comprising the mature polypeptide of SEQ ID NO: 2;

(ii) a cellobiohydrolase I comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2;

(iii) a cellobiohydrolase I encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1; and

(iv) a cellobiohydrolase I encoded by a polynucleotide that hybridizes under very high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 1 or the full-length complement thereof;

wherein the Aspergillus fumigatus cellobiohydrolase II or homolog thereof is selected from the group consisting of:

(i) a cellobiohydrolase II comprising the mature polypeptide of SEQ ID NO: 4;

(ii) a cellobiohydrolase II comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 4;

(iii) a cellobiohydrolase II encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 3; and

(iv) a cellobiohydrolase II encoded by a polynucleotide that hybridizes under very high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 3 or the full-length complement thereof;

wherein the Aspergillus fumigatus beta-glucosidase or homolog thereof is selected from the group consisting of:

(i) a beta-glucosidase comprising the mature polypeptide of SEQ ID NO: 6;

(ii) a beta-glucosidase comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 6;

(iii) a beta-glucosidase encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 5; and

(iv) a beta-glucosidase encoded by a polynucleotide that hybridizes under very high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 5 or the full-length complement thereof;

wherein the Aspergillus fumigatus GH61 polypeptide having cellulolytic enhancing activity or homolog thereof is selected from the group consisting of:

(i) a GH61 polypeptide having cellulolytic enhancing activity comprising the mature polypeptide of SEQ ID NO: 8;

(ii) a GH61 polypeptide having cellulolytic enhancing activity comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 8;

(iii) a GH61 polypeptide having cellulolytic enhancing activity encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 7; and

(iv) a GH61 polypeptide having cellulolytic enhancing activity encoded by a polynucleotide that hybridizes under very high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 7 or the full-length complement thereof;

wherein the Aspergillus fumigatus xylanase or homolog thereof is selected from the group consisting of:

(i) an Aspergillus fumigatus xylanase comprising the mature polypeptide of SEQ ID NO: 14, SEQ ID NO: 16, or SEQ ID NO: 18;

(ii) a xylanase comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 14, SEQ ID NO: 16, or SEQ ID NO: 18;

(iii) a xylanase encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 13, SEQ ID NO: 15, or SEQ ID NO: 17; and

(iv) a xylanase encoded by a polynucleotide that hybridizes under very high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 13, SEQ ID NO: 15, or SEQ ID NO: 17; or the full-length complement thereof;

wherein the Aspergillus fumigatus beta-xylosidase or homolog thereof is selected from the group consisting of:

(i) a beta-xylosidase comprising the mature polypeptide of SEQ ID NO: 20;

(ii) a beta-xylosidase comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 20;

(iii) a beta-xylosidase encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 19; and

(iv) a beta-xylosidase encoded by a polynucleotide that hybridizes under very high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 19 or the full-length complement thereof; and

wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.

2. The recombinant Trichoderma host cell of claim 1 , which further comprises one or more polynucleotides encoding one or more enzymes selected from the group consisting of: (i) an Aspergillus fumigatus endoglucanase I; (ii) an Aspergillus fumigatus endoglucanase II; and (iii) an Aspergillus fumigatus swollenin;

wherein the Aspergillus fumigatus endoglucanase I or homolog thereof is selected from the group consisting of:

(i) an endoglucanase I comprising the mature polypeptide of SEQ ID NO: 10;

(ii) an endoglucanase I comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 10;

(iii) an endoglucanase I encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 9; and

(iv) an endoglucanase I encoded by a polynucleotide that hybridizes under very high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 9 or the full-length complement thereof;

wherein the Aspergillus fumigatus endoglucanase II or homolog thereof is selected from the group consisting of:

(i) an Aspergillus fumigatus endoglucanase II comprising the mature polypeptide of SEQ ID NO: 12;

(ii) an endoglucanase II comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 12;

(iii) an endoglucanase II encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 11; and

(iv) an endoglucanase II encoded by a polynucleotide that hybridizes under very high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 11 or the full-length complement thereof;

wherein the Aspergillus fumigatus swollenin or homolog thereof is selected from the group consisting of:

(i) a swollenin comprising the mature polypeptide of SEQ ID NO: 22;

(ii) a swollenin comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 22;

(iii) a swollenin encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 21; and

(iv) a swollenin encoded by a polynucleotide that hybridizes under very high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 21 or the full-length complement thereof; and

wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.

3. The recombinant Trichoderma host cell of claim 1 , which is selected from the group consisting of Trichoderma harzianum, Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma reesei , and Trichoderma viride.

4. The recombinant Trichoderma host cell of claim 1 , which is Trichoderma reesei.

5. A method of producing an enzyme composition, comprising: cultivating the host cell of claim 1 under conditions conducive for production of the enzyme composition.

6. The method of claim 5 , further comprising recovering the enzyme composition.