Antibodies that bind integrin alpha-V beta-8
Provided herein are antibodies with high affinity for the β8 subunit of αvβ8.
1. An isolated antibody that specifically binds αvβ8, wherein the antibody has a heavy chain variable region comprising SEQ ID NO:8 and a light chain variable region comprising SEQ ID NO:9.
2. The isolated or humanized antibody of claim 1 , wherein the antibody is an scFv antibody.
3. The isolated antibody of claim 1 linked to a detectable label.
4. The isolated antibody of claim 1 , wherein the isolated antibody is humanized.
5. A pharmaceutical composition comprising the isolated antibody of claim 1 in a pharmaceutically acceptable excipient.
6. A method of reducing TGFβ signaling in an individual, comprising administering the pharmaceutical composition of claim 5 to the individual, thereby reducing TGFβ signaling in the individual.
7. The method of claim 6 , wherein the individual has at least one condition selected from the group consisting of inflammatory bowel disease (IBD), chronic obstructive pulmonary disease (COPD), asthma, and a pulmonary fibrotic disorder, and wherein reducing TGFβ signaling results in amelioration of the condition.
8. A method of detecting αvβ8 in a cell from an individual, comprising
contacting the cell from the individual with the isolated antibody of claim 1 , and
detecting binding of the isolated antibody to the cell.
9. The method of claim 8 , wherein the cells are fibroblasts, chondrocytes, or hepatic stellate cells.