Methods and materials for obtaining induced pluripotent stem cells
This document provides methods and materials involved obtaining induced pluripotent stem (iPS) cells. For example, methods and materials for increasing the efficiency for making iPS cells as well as methods and materials for selecting iPS cells are provided.
1. A method for augmenting production of induced pluripotent stem cells being produced from isolated somatic cells by contacting said isolated somatic cells with stemness transcription factors, wherein said stemness transcription factors comprise OCT3/4, SOX2, and KLF4, wherein said method comprises culturing said isolated somatic cells in medium comprising about 12.5 mM of glucose to produce said induced pluripotent stem cells.
2. The method of claim 1 , wherein said somatic cells are fibroblasts.
3. The method of claim 1 , wherein said stemness transcription factors further comprise c-MYC.