Encapsulated diagnostics and therapeutics in nanoparticles—conjugated to tropic cells and methods for their use
A therapeutic or diagnostic delivery vehicle is provided. The delivery vehicle may include one or more particles, such as microparticles, nanoparticles and stimuli-responsive particles, conjugated to a tropic cell that targets at least one pathological entity or site. In addition, a pharmaceutical composition is provided. The pharmaceutical composition may include, among other things, a particle conjugated to a tropic cell such as those discussed above and at least one diagnostic or therapeutic agent, such as those described herein. In some aspects, the tropic cell may target at least one pathological entity or site. Further, methods for diagnosing, monitoring or treating a pathological condition in a subject are provided. Such methods may include administering a therapeutically effective amount of the pharmaceutical composition to a subject.
1. A therapeutic delivery vehicle comprising a gold particle coated with a cationic thiol moiety internalized by a genetically modified neural stem cell, wherein the cationic thiol moiety is 11-mercaptoundecyltrimethylammonium bromide.
2. The therapeutic delivery vehicle of claim 1 , wherein the genetically modified neural stem cell is an immortalized neural stem cell.
3. The therapeutic delivery vehicle of claim 1 , wherein the genetically modified neural stem cell expresses cytosine deaminase, v-myc, or both cytosine deaminase and v-myc.
4. The therapeutic delivery vehicle of claim 1 , wherein the gold particle is a gold nanorod.
5. The therapeutic delivery vehicle of claim 1 , wherein the average longest dimension of the gold particle is about 41 nm.
6. The therapeutic delivery vehicle of claim 1 , wherein the gold particle is about 10 nm by about 41 nm.