ENA nucleic acid pharmaceuticals capable of modifying splicing of mRNA precursors
Oligonucleotides having a nucleotide sequence complementary to nucleotide numbers such as 2571-2607, 2578-2592, 2571-2592, 2573-2592, 2578-2596, 2578-2601 or 2575-2592 of the dystrophin cDNA (Gene Bank accession No. NM_004006.1) and therapeutic agents for muscular dystrophy comprising such oligonucleotides.
1. An oligonucleotide having the nucleotide sequence as shown in any one of SEQ ID NOS: 19, 20, 30, 41, 64, 66, or 87 in the SEQUENCE LISTING, or a pharmacologically acceptable salt thereof, wherein at least one sugar constituting the oligonucleotide is modified and/or at least one phosphate constituting the oligonucleotide is modified.
2. The oligonucleotide of claim 1 , wherein the oligonucleotide has the nucleotide sequence as shown in SEQ ID NO: 19 in the SEQUENCE LISTING, or a pharmacologically acceptable salt thereof, wherein at least one sugar constituting the oligonucleotide is modified and/or at least one phosphate constituting the oligonucleotide is modified.
3. The oligonucleotide of claim 1 , wherein the oligonucleotide has the nucleotide sequence as shown in SEQ ID NO: 20 in the SEQUENCE LISTING, or a pharmacologically acceptable salt thereof, wherein at least one sugar constituting the oligonucleotide is modified and/or at least one phosphate constituting the oligonucleotide is modified.
4. The oligonucleotide of claim 1 , wherein the oligonucleotide has the nucleotide sequence as shown in SEQ ID NO: 30 in the SEQUENCE LISTING, or a pharmacologically acceptable salt thereof, wherein at least one sugar constituting the oligonucleotide is modified and/or at least one phosphate constituting the oligonucleotide is modified.
5. The oligonucleotide of claim 1 , wherein the oligonucleotide has the nucleotide sequence as shown in SEQ ID NO: 41 in the SEQUENCE LISTING, or a pharmacologically acceptable salt thereof, wherein at least one sugar constituting the oligonucleotide is modified and/or at least one phosphate constituting the oligonucleotide is modified.
6. The oligonucleotide of claim 1 , wherein the oligonucleotide has the nucleotide sequence as shown in SEQ ID NO: 64 in the SEQUENCE LISTING, or a pharmacologically acceptable salt thereof, wherein at least one sugar constituting the oligonucleotide is modified and/or at least one phosphate constituting the oligonucleotide is modified.
7. The oligonucleotide of claim 1 , wherein the oligonucleotide has the nucleotide sequence as shown in SEQ ID NO: 66 in the SEQUENCE LISTING, or a pharmacologically acceptable salt thereof, wherein at least one sugar constituting the oligonucleotide is modified and/or at least one phosphate constituting the oligonucleotide is modified.
8. The oligonucleotide of claim 1 , wherein the oligonucleotide has the nucleotide sequence as shown in SEQ ID NO: 87 in the SEQUENCE LISTING, or a pharmacologically acceptable salt thereof, wherein at least one sugar constituting the oligonucleotide is modified and/or at least one phosphate constituting the oligonucleotide is modified.
9. The oligonucleotide or a pharmacologically acceptable salt thereof according to claim 1 , wherein at least one of sugar constituting the oligonucleotide is modified, the sugar constituting the oligonucleotide is D-ribofuranose, and the modification of the sugar is modification of the hydroxyl group at position 2′ of D-ribofuranose.
10. The oligonucleotide or a pharmacologically acceptable salt thereof according to claim 1 , wherein the modification of the sugar is 2′-O-alkylation and/or 2′-O,4′-C-alkylenation of the D-ribofuranose.
11. The oligonucleotide or a pharmacologically acceptable salt thereof according to claim 1 , wherein at least one of sugar constituting the oligonucleotide is modified, and said modification is selected from the group consisting of 2% O-methylation of D-ribofuranose, 2′-O-aminoethylation of D-ribofuranose, 2′-O-propylation of D-ribofuranose, 2′-O-allylation of D-ribofuranose, 2′-O-methoxyethylation of D-ribofuranose, 2′-O-butylation of D-ribofuranose, 2′-O-pentylation of D-ribofuranose, 2′-O-propargylation of D-ribofuranose, 2′-O,4′-C-ethylenation of D-ribofuranose, 2′-O,4′-C-methylenation of D-ribofuranose, 2′-O,4′-C-propylenation of D-ribofuranose, 2′-O,4′-C-tetramethylation of D-ribofuranose, 2′-O,4′-C-pentamethylation of D-ribofuranose, 3′-deoxy-3′-amino-2′-deoxy-D-ribofuranose, and 3′-deoxy-3′-amino-2′-deoxy-2′-fluoro-D-ribofuranose.
12. The oligonucleotide or a pharmacologically acceptable salt thereof according to claim 1 , wherein at least one of phosphate constituting the oligonucleotide is modified and the modification of the phosphate is thioation of the phosphate group.
13. The oligonucleotide or a pharmacologically acceptable salt thereof according to claim 1 , wherein at least one sugar constituting the oligonucleotide is modified and at least one phosphate constituting the oligonucleotide is modified.
14. The oligonucleotide or a pharmacologically acceptable salt thereof according to claim 1 , wherein at least one sugar constituting the oligonucleotide is modified.
15. The oligonucleotide or a pharmacologically acceptable salt thereof according to claim 1 , wherein at least one phosphate constituting the oligonucleotide is modified.