Nanoimprinting of silk fibroin structures for biomedical and biophotonic applications
The present invention provides for photonic nanoimprinted silk fibroin-based materials and methods for making same, comprising embossing silk fibroin-based films with photonic nanometer scale patterns. In addition, the invention provides for processes by which the silk fibroin-based films can be nanoimprinted at room temperature, by locally decreasing the glass transition temperature of the silk film. Such nanoimprinting process increases high throughput and improves potential for incorporation of silk-based photonics into biomedical and other optical devices.
1. A silk fibroin-based biopolymer film, comprising:
features formed on a surface of the film and arranged in a pattern that
(a) corresponds to a master pattern, and
(b) substantially lacks artifacts
wherein the patterned features include one or more transverse features with a minimum dimension of about 20 nm or less, which transverse features are reproduced relative to the master pattern with a standard deviation of ±10 nm.
2. The silk fibroin-based biopolymer film of claim 1 , wherein the patterned features have at least one minimum dimension of about 50 nm or less.
3. The silk fibroin-based biopolymer film of claim 1 , wherein the patterned features have at least one minimum dimension of about 20 nm or less.
4. The silk fibroin-based biopolymer film of claim 1 , wherein the pattern on the surface comprises a 3-dimensional structure.
5. The silk fibroin-based biopolymer film of claim 1 , wherein the pattern on the surface is a template for at least one selected from the group consisting of a lens, a microlens array, and optical grating, a pattern generator, a beam reshaper, and combinations thereof.
6. The silk fibroin-based biopolymer film of claim 1 , wherein one or more active agents are embedded in or immobilized on the silk fibroin-based biopolymer film.
7. The silk fibroin-based biopolymer film of claim 1 , wherein the active agents are cells, proteins, nucleic acids, antibiotics, antibodies, enzymes, or combinations thereof.
8. An optical device comprising the silk fibroin-based biopolymer film of claim 1 .
9. The optical device of claim 8 , wherein the optical device is a biophotonic sensor.
10. The optical device of claim 8 , wherein the optical device is an optofluidic device.
11. The silk fibroin-based biopolymer film of claim 1 , wherein the silk fibroin-based biopolymer film is integrated on an endface of an optical fiber.
12. The silk fibroin-based biopolymer film of claim 11 , wherein the pattern on the surface forms an optical component.
13. The silk fibroin-based biopolymer film of claim 11 , wherein the silk fibroin-based biopolymer film is incorporated with or functionalized with one or more bioactive agents.
14. The silk fibroin-based biopolymer film of claim 1 , wherein the artifact is uneven edges in the features formed into the surface of the film.
15. The silk fibroin-based biopolymer film of claim 1 , wherein the artifact is extra depth in the features formed into the surface of the film.