Systems and devices for analysis of samples
Systems and methods for analysis of samples, and in certain embodiments, microfluidic sample analyzers configured to receive a cassette containing a sample therein to perform an analysis of the sample are described. The microfluidic sample analyzers may be used to control fluid flow, mixing, and sample analysis in a variety of microfluidic systems such as microfluidic point-of-care diagnostic platforms. Advantageously, the microfluidic sample analyzers may be, in some embodiments, inexpensive, reduced in size compared to conventional bench top systems, and simple to use. Cassettes that can operate with the sample analyzers are also described.
1. A device comprising:
a first component comprising a first channel formed in a first material and including at least a first port, the first channel including at least one portion having a cross-sectional dimension greater than 200 microns, wherein the first material has a water vapor permeability of less than about 0.05 g·mm/m 2 ·d;
a second component comprising a second channel formed in a second material and including at least a second port, the second channel including at least one portion having a cross-sectional dimension less than 200 microns, wherein the second material has an optical transmission of greater than 90% between 400 nm and 800 nm wavelengths of light;
a fluidic connector that can be connected to the first and second components, the fluid connector comprising a fluid path including a fluid path inlet and a fluid path outlet, wherein upon connection, the fluid path inlet fluidically connects to the first port and the fluid path outlet fluidically connects to the second port to allow fluid communication between the first and second channels,
wherein the first and second channels are not in fluid communication with one another absent connection via the fluidic connector,
wherein the first and second components are substantially planar and lie on top of one another, and
wherein both the first port and the second port are accessible from a top surface of the first component or the second component.
2. A device as in claim 1 , wherein the first component comprises a feature complementary to a feature of the second component so as to form a non-fluidic connection between the first and second components.
3. A device as in claim 1 , wherein the first and second components are integrally connected to one another.
4. A device as in claim 1 , wherein the first and second components are reversibly attached to one another.
5. A device as in claim 1 , wherein the at least one portion of the first channel of the first component is substantially enclosed by the second component, and/or the at least one portion of the second channel of the second component is substantially enclosed by the first component.
6. A device as in claim 1 , wherein the at least one portion of the first channel of the first component is substantially enclosed by a first cover that is not the second component, and/or wherein the at least one portion of the second channel of the second component is substantially enclosed by a second cover that is not the first component.
7. A device as in claim 6 , wherein the first and/or second cover is a tape.
8. A device as in claim 1 , wherein the first material is different from the second material.
9. A device as in claim 1 , wherein the first material has a water vapor permeability between about 0.01 g·mm/m 2 ·d and about 0.4 g·mm/m 2 ·d.
10. A device as in claim 1 , wherein the first material has a higher melting temperature than the second material.
11. A device as in claim 1 , wherein the second material has a melting temperature of less than about 100° C.
12. A device as in claim 1 , wherein the second material has an optical transmission of greater than 95% between 400 nm and 800 nm wavelengths of light.
13. A device as in claim 1 , wherein the first material has an optical transmission of less than 10% between 400 nm and 800 nm wavelengths of light.
14. A device as in claim 1 , wherein the first material comprises a cyclo-olefin copolymer.
15. A device as in claim 1 , wherein the second material comprises polystyrene, PMMA, or a cyclo-olefin copolymer.
16. A device as in claim 1 , wherein the at least one portion of the first channel has a root mean square surface roughness of greater than about 5 microns, and the at least one portion of the second channel has a root mean square surface roughness of less than about 5 microns.
17. A device as in claim 1 , comprising a first reagent disposed in the first channel, wherein the first port of the first channel is sealed prior to first use so as to store the first reagent in the first channel, wherein the first reagent is a liquid.
18. A device as in claim 1 , comprising a second reagent disposed in the second channel, wherein the second reagent is substantially dried and is adsorbed to a surface of the at least one portion of the second channel.
19. A device as in claim 1 , wherein the second component comprises a reaction area in fluid communication with the second channel.
20. A device as in claim 19 , wherein the reaction area comprises at least one meandering channel region.
21. A device as in claim 19 , wherein the reaction area comprises at least two meandering channel regions connected in series.
22. A device as in claim 1 , comprising an alignment element associated with the first or second components, extending from one of the first or second components and comprising a cavity constructed and arranged to receive and engage the fluidic connector and thereby position the connector in a predetermined, set configuration relative to the first or second components.
23. A device as in claim 1 , wherein the fluid path of the fluid connector has a length of at least 2 cm.
24. A device as in claim 1 , wherein each of the first and second components comprises at least one portion having a thickness of at least 2.5 mm.
25. A device as in claim 1 , wherein each of the first and second components comprises at least a portion having a thickness of at least 2.5 mm, and wherein at least one of the first and second components comprises at least one portion having a thickness of less than 2.5 mm.
26. A device as in claim 1 , wherein both the first port and the second port extend above a top surface of the first component or the second component.