Compositions and methods for lipid production
Described herein, inter alia, are compositions, oleagnious organisms, and methods useful for producing lipids, lipid precursors, and/or oleochemicals.
1. A method of producing a lipid, lipid precursor, or oleochemical comprising:
a) culturing a genetically modified yeast cell in a growth medium; and
b) isolating said lipid, lipid precursor, or oleochemical,
wherein the dry weight of said genetically modified yeast cell comprises greater than 60% wt/wt lipids, lipid precursors, and oleochemicals; and
wherein said genetically modified yeast cell comprises (i) a recombinant acyl-CoA:diacylglycerol acyltransferase 1 (DGA1) gene and a UGA2 succinate semialdehyde dehydrogenase (UGA2) gene comprising a mutation, wherein said mutation results in a loss of function of succinate semialdehyde dehydrogenase of 20% or more compared to succinate semialdehyde dehydrogenase encoded by UGA2 without the mutation; or (ii) a recombinant acyl-CoA:diacylglycerol acyltransferase 2 (DGA2) gene and a UGA2 succinate semialdehyde dehydrogenase (UGA2) gene comprising a mutation, wherein said mutation results in a loss of function of succinate semialdehyde dehydrogenase of 20% or more compared to succinate semialdehyde dehydrogenase encoded by UGA2 without the mutation.
2. The method of claim 1 , wherein said growth medium comprises a majority carbon source selected from the group consisting of glucose, glycerol, xylose, fructose, mannose, ribose, sucrose, and lignocellulosic biomass.
3. The method of claim 1 , wherein said growth medium comprises lignocellulosic biomass as the majority carbon source.
4. The method of claim 1 , wherein said growth medium comprises cobalt, iron, magnesium, potassium, zinc, nickel, molybdenum, manganese, copper, or boron.
5. The method of claim 1 , wherein said growth medium comprises 5.77×10 −5 M to 1.73×10 −4 M cobalt, 0.001 M to 0.003 M magnesium, 4.52×10 −5 M to 1.35×10 −4 M potassium, 4.05×10 −5 M to 1.22×10 −4 zinc, 3.55×10 −5 M to 1.06×10 −4 manganese, 9.07×10 −5 M to 2.91×10 −4 boron, 3.76×10 −5 M to 1.10×10 −5 molybdenum, 2.28×10 −5 M to 6.84×10 −5 nickel, 3.60×10 −5 M to 1.08×10 −4 iron, or 4.70×10 −5 M to 1.41×10 −4 copper.
6. The method of claim 1 , wherein said genetically modified yeast cell comprises a recombinant Lipid synthesis regulator (MGA2) gene, a genetically modified Lipid synthesis regulator (MGA2) gene, a recombinant Leucine Biosynthesis gene (LEU2), a genetically modified multifunctional enzyme (MFEI) gene, a genetically modified PEX10 Transcription Factor (PEX10) gene or a recombinant AMP Deaminase (AMPD) gene.
7. The method of claim 1 , wherein said genetically modified yeast cell comprises a genetically modified multifunctional enzyme (MFE1) gene and a genetically modified PEX10 Transcription Factor (PEX10) gene.
8. The method of claim 1 , wherein said genetically modified yeast cell comprises a recombinant Leucine Biosynthesis gene (LEU2), a genetically modified multifunctional enzyme (MFEI) gene and a genetically modified PEX10 Transcription Factor (PEX10) gene.
9. The method of claim 1 , wherein said genetically modified yeast cell comprises a genetically modified multifunctional enzyme (MFEI) gene, a genetically modified PEX10 Transcription Factor (PEX10) gene and a recombinant AMP Deaminase (AMPD) gene.
10. The method of claim 1 , wherein said genetically modified yeast cell comprises a recombinant Leucine Biosynthesis gene (LEU2), a genetically modified multifunctional enzyme (MFEI) gene, a genetically modified PEX10 Transcription Factor (PEX10) gene and a recombinant AMP Deaminase (AMPD)) gene.