Photobioreactor
Provided herein is a transgenic bacteria engineered to accumulate carbohydrates, for example disaccharides. Also provided is a photobioreactor for cultivating photosynthetic microorganisms comprising a non-gelatinous, solid cultivation support suitable for providing nutrients and moisture to photosynthetic microorganisms and a physical barrier covering at least a portion of the surface of the cultivation support. Devices for the large scale and continuous cultivation of photosynthetic microorganisms incorporating photobioreactors and methods of use are disclosed. Also disclosed are methods of producing fermentable sugar from photosynthetic microorganisms using a photobioreactor of the invention.
1. A photobioreactor comprising:
a solid cultivation support, wherein the solid cultivation support comprises a non-gelatinous, textured surface suitable for culturing photosynthetic microorganisms;
a physical barrier; and
a volume of air;
wherein,
the physical barrier is disposed over at least a portion of the solid cultivation support; and
the volume of air is between the physical barrier and the solid cultivation support.
2. The photobioreactor of claim 1 , wherein the photobioreactor comprises a plurality of cultivation supports.
3. The photobioreactor of claim 1 , wherein the solid cultivation support is suitable for adherence of a photosynthetic microorganism.
4. The photobioreactor of claim 1 , wherein the solid cultivation support is suitable for culturing photosynthetic microorganisms at a density of at least about 50 grams of dry biomass per liter equivalent.
5. The photobioreactor of claim 1 , further comprising a spray device for distributing a liquid medium over the solid cultivation support.
6. The photobioreactor of claim 1 , wherein the solid cultivation support has a sheet shape and depth of the solid cultivation support that is substantially less than length and width of the solid cultivation support.
7. The photobioreactor of claim 1 , wherein the solid cultivation support comprises:
(i) a fabric comprising a natural, modified natural, or synthetic fiber, or a combination thereof;
(ii) a fabric comprising a woven fabric, a knitted fabric, a felt, a mesh of cross-linked fiber polymers, or a combination thereof;
(iii) a fabric comprising natural fibers selected from the group consisting of cotton, wool, hemp, tree fiber, other cellulosic fibers, and combinations thereof;
(iv) a fabric comprising modified natural fibers selected from the group consisting of nitrocellulose, cellulose acetate, cellulose sulfonate, crosslinked starches, and combinations thereof;
(v) a fabric comprising synthetic fibers selected from the group consisting of polyester, polyacrylate, polyamine, polyamide, polysulfone, and combinations thereof;
(vi) a material having loops;
(vii) a material having loops, the material being terry cloth; or
(viii) a combination thereof.
8. The photobioreactor of claim 1 , wherein the solid cultivation support further comprises a coating of a moisture absorbent polymer selected from the group consisting of agar, polyacrylate, polyamide, polyamine, polyethylene glycol, modified starches, and combinations thereof.
9. The photobioreactor of claim 1 , further comprising water, nutrients, or a combination thereof on or within the solid cultivation support.
10. The photobioreactor of claim 1 , wherein the solid cultivation support comprises: (i) a flexible material, (ii) a rigid material, or (iii) flexibly connected rigid portions, wherein the rigid portions are comprised of a rigid material.
11. The photobioreactor of claim 1 , wherein
the cultivation support comprises at least two layers, a first layer adjacent to a second layer, wherein material of the at least two layers is the same material or different materials; and
optionally, the first layer comprises a high surface area growth material and the second layer comprises a fluid permeable type material.
12. The photobioreactor of claim 1 , further comprising a suspension element, wherein the solid cultivation support is suspended from the suspension element.
13. The photobioreactor of claim 12 , wherein the solid cultivation support is suspended non-horizontally from the suspension element.
14. The photobioreactor of claim 12 , wherein the solid cultivation support is suspended substantially vertically from the suspension element.
15. The photobioreactor of claim 12 , wherein the suspension element comprises one or more attachment points for attaching the photobioreactor to a structure.
16. The photobioreactor of claim 1 , wherein the physical barrier is releasably sealed so as to enclose the solid cultivation support.
17. The photobioreactor of claim 1 , wherein the physical barrier, or a portion thereof, is a flexible physical barrier.
18. The photobioreactor of claim 1 , wherein the physical barrier, or a portion thereof, is (i) substantially impermeable to solid particulate or liquid and (ii) partially or substantially permeable to gas or vapor.
19. The photobioreactor of claim 1 , wherein the physical barrier, or a portion thereof, is substantially transparent to actinic irradiation.
20. The photobioreactor of claim 1 , wherein the physical barrier comprises a first barrier portion and a second barrier portion, wherein the first barrier portion and the second barrier portion are different with respect to one or more of liquid permeability, gas permeability, or transparency to actinic radiation.
21. The photobioreactor of claim 1 , further comprising a source of actinic radiation that is (i) external to the physical barrier and the physical barrier is substantially transparent to actinic radiation or (ii) between the solid cultivation support and the physical barrier.
22. The photobioreactor of claim 1 , further comprising at least one of a fluid supply system, nutrient supply system, gas supply system, or microorganism supply system.
23. The photobioreactor of claim 1 , further comprising photosynthetic microorganisms in or on the solid cultivation support.
24. The photobioreactor of claim 23 , wherein the photosynthetic microorganisms comprise a transgenic photosynthetic microorganism cell, the cell comprising an artificial DNA construct comprising, as operably associated components in the 5′ to 3′ direction of transcription,
a promoter functional in the photosynthetic microorganism cell,
a polynucleotide comprising a nucleotide sequence encoding a polypeptide having a disaccharide biosynthetic activity selected from the group consisting of a disaccharide phosphate synthase and a disaccharide phosphate phosphatase, and
a transcriptional termination sequence;
wherein,
the transgenic photosynthetic microorganism cell accumulates increased levels of the disaccharide compared to a photosynthetic microorganism cell not comprising the DNA construct, and
the cell is adhered to said portion of the surface of the cultivation support.
25. A device for cultivating photosynthetic microorganisms, comprising:
the photobioreactor of claim 1 ; and
a structure;
wherein
the photobioreactor is attached to the structure; and
the solid cultivation support is oriented non-horizontally.
26. The device of claim 1 , further comprising:
at least one of a fluid supply system, nutrient supply system, gas supply system, or microorganism supply system;
wherein the fluid supply system, nutrient supply system, gas supply system, or microorganism supply system is operably connected to the photobioreactor.
27. The device of claim 1 , wherein the photobioreactor is suspended from the structure and the structure is substantially covered by the physical barrier.