Patent
A cyanobacterial host cell, Cyanobacterium sp., that harbors at least one recombinant gene for the production of a chemical compounds is provided, as well as vectors derived from an endogenous plasmid isolated from the cell.
1. A genetically enhanced ethanologenic Cyanobacterium sp. ABICyanol host cell comprising Cyanobacterium sp. ABICyanol deposited in the American Type Culture Collection (ATCC) under ATCC accession number PTA-13311 comprising a genetically enhanced plasmid derived from a plasmid that is endogenous to Cyanobacterium sp. ABICyanol, and wherein said plasmid comprises a heterologous alcohol dehydrogenase gene and a heterologous pyruvate decarboxylase gene, and
wherein said alcohol dehydrogenase gene is operably linked to a constitutive promoter, and
wherein said pyruvate decarboxylase gene is operably linked to an inducible promoter, and
wherein said Cyanobacterium sp. ABICyanol can tolerate salinities ranging up to about 35 practical salinity units, and
can tolerate temperatures ranging up to about 55 degrees Celsius, and
can tolerate dissolved oxygen concentrations ranging up to about 1000 μmol/L, and
can tolerate ethanol. concentrations ranging up to about one percent for 16 weeks, and
wherein production of ethanol by said ethanologenic Cyanobacterium sp. ABICyanol host cell is from about 0.017 percent ethanol (vol/vol) per OD 750 per day to about 0.031 percent ethanol (vol/vol) per OD 750 per day.
2. The host cell of claim 1 wherein said constitutive promoter and said inducible promoter are endogenous to Cyanobacterium sp. ABICyanol.
3. The host cell of claim 1 wherein said constitutive promoter is heterologous to Cyanobacterium sp. ABICyanol.