Method to formulate T-cells
A method for formulating T-cells for use as a medicant comprises suspending T-cells with cross-linked surface moieties in a media suitable for infusion. The cross-linked T-cells are packaged in a container suitable for administration to a patient.
1. A method of formulating T-cells for use as a medicant comprising:
suspending T-cells with cross-linked surface moieties in a media suitable for infusion; and
packaging the cross-linked T-cells in a container suitable for administration to a patient.
2. The method of claim 1 wherein the cross-linked surface moieties are CD3 and CD28.
3. The method of claim 1 wherein the T-cells are Th1 cells.
4. The method of claim 2 wherein the cross-linking is through anti-CD3 and anti-CD28 monoclonal antibodies.
5. The method of claim 1 wherein the container is a syringe.
6. The method of claim 1 wherein cross-linking of the T-cell surface moieties causes activation of the T-cells.
7. The method of claim 6 wherein the T-cells are activated through one or more agents which have reactivity for the T-cell surface moieties and capable of delivering activation signals upon cross-linking.
8. The method of claim 1 wherein the T-cells are cross-linked through a first material and one or more second materials, wherein the first material cross-links the one or more second materials and the second materials have reactivity to the surface moieties on the T-cells.