Simultaneous dehydration and staining of tissue for deep imaging
A biopsy-sized tissue sample is stained for quick imaging. A significant amount of permeation enhancer is included in a mixed solution of permeant enhancer, fixative or dehydrant, and one or two fluorescent dyes to simultaneously dehydrate and dye the tissue sample. The permeation enhancer, e.g., 10% to 50% in the mixed solution, achieves an image of dyed tissue in the contacted tissue sample at a depth of at least 200 um within no more than 1.5 hours. One of the fluorescent dyes is a fluorescent nuclear dye such as DAPI, SYTOX green, acridine orange, propidium iodide, or a Hoechst dye. The other fluorescent dye is a fluorescent protein dye such as eosin or rhodamine B. The tissue sample is cleared with a clearing agent having a refractive index of at least 1.4[R2], e.g., using BABB. The mixed solution may further include Chloroform or other morphology preservative.
1. A method of processing a tissue sample for deep imaging, the method comprising:
obtaining a tissue sample;
contacting the tissue sample with a fixative solution at 45° C., the fixative solution comprising methacarn, 10 μM DAPI, and 0.5% by volume eosin for about 1 hour; and
transferring the tissue sample directly to a solution of 100% benzyl alcohol and benzyl benzoate (BABB) for about 30 minutes.
2. The method of processing a tissue sample for deep imaging according to claim 1 , further comprising the step of imaging the tissue sample in the solution of 100% BABB to produce an image of the tissue sample at the depth of 100 nm to 2 cm.
3. The method of processing a tissue sample for deep imaging according to claim 1 , wherein the fixative solution further comprises a red blood cell lysing agent.
4. The method of processing a tissue sample for deep imaging according to claim 1 , wherein:
the tissue sample obtained is core needle biopsy-sized.
5. The method of processing a tissue sample for deep imaging according to claim 1 , wherein the fixative solution comprises:
about 10% of a morphology preservative.
6. The method of processing a tissue sample for deep imaging according to claim 1 , further comprising fixing the tissue sample in formalin before contacting the tissue sample with the fixative solution.
7. The method of processing a tissue sample for deep imaging according to claim 6 , further comprising fixing the tissue sample in formalin for a period of time from 20 minutes to 4 weeks before contacting the tissue sample with the fixative solution.