Embryo culture methods and media
Improved methods of culturing embryos in media having amounts of lactate that have not previously been recognized as beneficial for embryo development. Also, compositions, devices and kits related to the same.
1. A method of culturing a human embryo in vitro, the method comprising culturing a human embryo in a medium comprising: a starting lactate concentration of between about 0.3 mM and about 1 mM, and a glucose concentration of less than about 2 mM, wherein the medium does not contain serum, wherein the medium is a sequential medium or a medium that is replenished at least once during the culturing of the embryo.
2. The method of claim 1 , wherein the starting lactate concentration is about 1 mM.
3. The method of claim 1 , further comprising culturing the embryo in an incubator.
4. The method of claim 3 , wherein the incubator comprises an imaging system to capture one or more images of the embryo during culturing.
5. The method of claim 4 , wherein the one or more images can be captured without moving the embryo.
6. The method of claim 1 , wherein the medium is a modified version of any of the following: P-1 medium, G1 medium, G2 Medium, a Human Tubal Fluid (HTF) medium with or without glucose or phosphate, modified HTF medium, Whitten's Medium, Ham's F-10 Medium, or KSOM medium, with or without protein supplementation.
7. The method of claim 1 , wherein said embryo is from a patient suffering from infertility or undergoing fertility treatment.
8. The method of claim 1 , wherein said embryo is cultured for about 1 day to about 7 days.
9. The method of claim 8 , wherein the embryo is cultured for about 3 days to about 6 days.
10. The method of claim 9 , wherein the embryo is cultured until it reaches an 8-cell stage or a blastocyst stage.
11. The method of claim 1 , further comprising implanting said embryo into a female patient.
12. A composition for culturing a human embryo, comprising an embryo culture medium suitable for culturing a human embryo and that is modified to have:
(i) a lactate concentration of between about 0.3 mM and about 1 mM,
(ii) a glucose concentration of less than about 2 mM,
(iii) human serum albumin; and
wherein the embryo culture medium does not contain serum.
13. The method of claim 1 , wherein the medium further comprises human serum albumin.
14. The composition of claim 12 , wherein the lactate concentration is about 1 mM.
15. The composition of claim 12 , further comprising at least one additional energy substrate.
16. The composition of claim 15 , wherein the energy substrate is pyruvate.
17. The composition of claim 16 , wherein the pyruvate is present in the composition at a concentration of between about 0.1 mM to about 3 mM.