Sensor housing and reagent chemistry

1. A sensor for use with a cuvette, the sensor comprising:

a porous substrate having an analysis chemistry reagent; and

a sensor housing comprising a sensor cap and a sensor base, wherein:

the sensor cap comprises:

a first channel; and

a first interlocking region, the first channel having a first diameter at the first interlocking region; and

the sensor base comprises:

a second interlocking region to mate with the first interlocking region;

a second channel adjacent the second interlocking region, the second channel holding the porous substrate and in fluid communication with the first channel, the second channel having a second diameter greater than the first diameter;

a third channel coupled to and in fluid communication with the second channel, the third channel having a third diameter smaller than both the first diameter and the second diameter; and,

an outer housing wall to fit over and around a perimeter of an upper chamber of the cuvette.

2. The sensor of claim 1 , wherein the sensor base further comprises:

a nozzle, in fluid connection with the third channel and insertable into an opening of the cuvette to direct liquid flow into a lower chamber of the cuvette.

3. The sensor of claim 2 , wherein the sensor base further comprises:

an inner housing wall coupled to and spaced apart from the nozzle and the outer housing wall, the inner housing wall to fit within the upper chamber of the cuvette.

4. The sensor of claim 1 , wherein the coupling of the third channel and the second channel form a second channel base, and the porous substrate is held in between a lower surface of the sensor cap at the first interlocking region and the second channel base, and further in contact with the third channel.

5. The sensor of claim 1 , wherein the first diameter of the first channel at the first interlocking region is substantially at least about 1.5 times the third diameter of the third channel at the porous substrate and the second diameter of the second channel is substantially at least about 2.0 times the third diameter at the porous substrate.

6. The sensor of claim 1 , wherein the porous substrate is a frit and the analysis chemistry reagent comprises a nucleic acid enzyme and a fluorophore.

7. The sensor of claim 1 , wherein:

the sensor cap further comprises a sensor cap baffle and baffle flares disposed on opposing sides of the sensor cap baffle; and

wherein the first channel extends axially through the sensor cap, the second and third channels extend axially through the sensor base, and an upper portion of the first channel of the sensor cap is to receive and removably couple tightly to a syringe having a sample for injection of the sample into the sensor housing.

8. The sensor of claim 1 , wherein the sensor housing is color-coded using a plurality of colors, each color of the plurality of colors to signify a selected analyte for detection in a sample or in a calibration sample.

9. The sensor of claim 1 , wherein the first interlocking region is a receptive interlocking region and the second interlocking region is an insertive interlocking region.

10. The sensor of claim 1 , wherein the sensor cap and the sensor base have been snapped or welded together.

11. A sensor for use with a cuvette, the sensor comprising:

a sensor housing comprising a sensor cap and a sensor base, wherein:

the sensor cap comprises:

a first axial channel; and

a first interlocking region, the first axial channel having a first diameter at the first interlocking region; and

the sensor base comprises:

a second interlocking region to mate with the first interlocking region;

a second axial channel adjacent the second interlocking region, the second axial channel holding the porous substrate and in fluid communication with the first axial channel, the second axial channel having a second diameter greater than the first diameter;

a third axial channel coupled to the second axial channel to form a lower rim in the second axial channel and in fluid communication with the second axial channel, the third axial channel having a third diameter smaller than both the first diameter and the second diameter;

a nozzle, in fluid connection with the third channel and insertable into an opening of the cuvette to direct liquid flow into a lower chamber of the cuvette;

an outer housing wall to fit over and around a perimeter of an upper chamber of the cuvette; and

an inner housing wall coupled to and spaced apart from the nozzle and the outer housing wall, the inner housing wall to fit within the upper chamber of the cuvette;

and

a porous substrate held in the second axial channel between a lower surface of the sensor cap at the first interlocking region and the lower rim of the second axial channel of the sensor base and in fluid communication with the first and third axial channels, the porous substrate having an analysis chemistry reagent.

12. The sensor of claim 11 , wherein the coupling of the third axial channel and the second axial channel form a second channel base, and the porous substrate is held in between a lower surface of the sensor cap at the first interlocking region and the second channel base, and further in contact with the third axial channel.

13. The sensor of claim 11 , wherein the first diameter of the first axial channel at the first interlocking region is substantially at least about 1.5 times the third diameter of the third axial channel at the porous substrate and the second diameter of the second axial channel is substantially at least about 2.0 times the third diameter at the porous substrate.

14. The sensor of claim 11 , wherein the porous substrate is a frit and the analysis chemistry reagent comprises a nucleic acid enzyme, a fluorophore, and a saccharide.

15. The sensor of claim 11 , wherein:

the sensor cap further comprises a sensor cap baffle and baffle flares disposed on opposing sides of the sensor cap baffle; and

wherein an upper portion of the first axial channel of the sensor cap is to receive and removably couple tightly to a syringe having a sample for injection of the sample into the sensor housing.

16. The sensor of claim 11 , wherein the sensor housing is color-coded using a plurality of colors, each color of the plurality of colors to signify a selected analyte for detection in a sample or in a calibration sample.

17. A sensor for use with a cuvette, the sensor comprising:

a porous substrate having an analysis chemistry reagent, the analysis chemistry reagent comprising a nucleic acid enzyme, a fluorophore, and a saccharide; and

a sensor housing comprising: a sensor cap and a sensor base, wherein:

the sensor cap comprises:

a first axial channel, an upper portion of the first axial channel removably coupleable to a syringe having a sample for injection of the sample into the sensor housing: and

a first interlocking region, the first axial channel having a first diameter at the first interlocking region;

a sensor cap baffle; and

baffle flares disposed on opposing sides of the sensor cap baffle

the sensor base comprises:

a second interlocking region to mate with the first interlocking region;

a second axial channel adjacent the second interlocking region, the second axial channel holding the porous substrate and in fluid communication with the first axial channel, the second channel having a second diameter greater than the first diameter;

a third axial channel coupled to and in fluid communication with the second axial channel, the third axial channel having a third diameter smaller than both the first diameter and the second diameter;

a nozzle, in fluid connection with the third axial channel and insertable into an opening of the cuvette to direct liquid flow into a lower chamber of the cuvette;

an outer housing wall to fit over and around a perimeter of an upper chamber of the cuvette; and

an inner housing wall coupled to and spaced apart from the nozzle and the outer housing wall, the inner housing wall to fit within the upper chamber of the cuvette; and

wherein the porous substrate is held in the second axial channel between a lower surface of the sensor cap at the first interlocking region and a lower rim of the second axial channel of the sensor base and in fluid communication with the first and third axial channels; and

wherein the sensor housing is color-coded using a plurality of colors, each color of the plurality of colors to signify a selected analyte for detection in a sample or in a calibration sample.