Compositions and methods related to isolated endophytes
Described herein are compositions and methods related to isolated Trichoderma harzianum and strains thereof.
1. A method for promoting abiotic stress tolerance and/or enhancing plant growth or seed germination under abiotic stress, the method comprising:
manually or mechanically inoculating a plant or a plant seed with an isolated Trichoderma harzianum strain ThLm1 fungus deposited at the Agricultural Research Service Culture Collection under Patent Deposit Designation number NRRL 50846, or a conidium or asexual progeny thereof.
2. The method of claim 1 , wherein the abiotic stress tolerance is drought tolerance, salt tolerance, reduced nutrient tolerance, and/or temperature tolerance.
3. The method of claim 1 , wherein the enhanced plant growth or seed germination under abiotic stress comprises an increase in size, extent of root development, germination rate of seeds, chlorophyll content/level, photosynthetic efficiency, yield or mass.
4. The method of claim 1 , wherein manually or mechanically inoculating the plant comprises colonizing a root and/or stem of the plant with the Trichoderma harzianum strain ThLm1 fungus or a conidium or asexual progeny thereof.
5. The method of claim 1 , wherein the method further comprises growing the plant or plant seed.
6. The method of claim 5 , wherein the plant or plant seed is grown in soil characterized by high salinity, low moisture, and/or low nutrient content, or wherein the plant or plant seed is grown in water characterized by high salinity and/or low nutrient content, or wherein the plant or plant seed is grown or germinated at an average temperature at or above 35 degrees Celsius or at or below 15 degrees Celsius.
7. The method of claim 1 , wherein the plant or plant seed is a crop plant or crop plant seed, or an ornamental plant or ornamental plant seed.
8. The method of claim 1 , wherein the isolated Trichoderma harzianum strain ThLm1 fungus or conidium or asexual progeny thereof is in a composition that is formulated as a liquid or a powder.
9. The method of claim 7 , wherein the crop plant or crop plant seed is watermelon, tomato, corn, wheat, soybean, cucurbits, peppers, leafy greens, barley, cotton, beans, peas, tubers, berries, woody plants or rice, or a seed thereof.
10. The method of claim 1 , wherein the plant or plant seed is an ornamental, such as Rosaceae, Liliaceae, Azalea, Rhododendron, Poaceae, or Chrysanthemum.
11. The method of claim 1 , wherein the plant or plant seed is previously, concurrently and/or subsequently treated with a fungicide and/or insecticide.