Plants having enhanced nitrogen efficiency
View Patent ↗A transgenic plant comprising a polynucleotide encoding a nitrogen utilization protein operably linked to a PBpr1 promoter is provided. The transgenic plant exhibits increased nitrogen use efficiency, increased biomass and/or increased seed yield. Seeds from such transgenic plants, genetic constructs to prepare such plants, methods of generating and growing transgenic plants are also provided.
1. A transgenic monocot plant, plant cell or plant part comprising a polynucleotide encoding an alanine aminotransferase comprising the amino acid sequence of SEQ ID No. 3 operably linked to a PBpr1 promoter comprising the nucleotide sequence of SEQ ID No. 1.
2. The transgenic plant, plant cell or plant part according to claim 1 , wherein the plant is wheat, maize, rice, or barley.
3. The transgenic monocot plant, plant cell or plant part according to claim 1 , wherein the polynucleotide encoding an alanine aminotransferase comprises the nucleotide sequence of SEQ ID No. 2.
4. A seed obtained from the transgenic monocot plant according to claim 1 , the seed comprising a polynucleotide encoding an alanine aminotransferase comprising the amino acid sequence of SEQ ID No. 3 operably linked to a PBpr1 promoter comprising the nucleotide sequence of SEQ ID No. 1.
5. A genetic construct comprising a polynucleotide encoding an alanine aminotransferase comprising the amino acid sequence of SEQ ID No. 3 operably linked to a PBpr1 promoter comprising the nucleotide sequence of SEQ ID No. 1.
6. The genetic construct according to claim 5 , wherein the polynucleotide encoding an alanine aminotransferase comprises the nucleotide sequence of SEQ ID No. 2.
7. A method of generating a monocot plant having increased nitrogen use efficiency, increased biomass, increased seed yield, increased tillering or a combination thereof, comprising:
transforming a monocot plant cell with the genetic construct according to claim 5 , and growing the transformed monocot plant cell to produce a monocot plant that expresses the alanine aminotransferase under control of the PBpr1 promoter, wherein the monocot plant has increased nitrogen use efficiency, increased biomass increased seed yield, increased tillering or a combination thereof compared to a wild-type monocot plant grown under identical conditions.
8. A method for producing a monocot plant having increased nitrogen use efficiency, increased biomass, increased seed yield, increased tillering or a combination thereof, comprising:
growing a monocot plant from a seed or regenerating a monocot plant from a plant part, wherein the plant part or seed comprises the genetic construct according to claim 5 ,
wherein the monocot plant has increased nitrogen use efficiency, increased biomass, increased seed yield, increased tillering or a combination thereof compared to a wild-type monocot plant grown under identical conditions.
9. The method of claim 8 , further comprising providing a fertilizer to the monocot plant or the habitat of the monocot plant.
10. The method of claim 9 , wherein the fertilizer is a nitrogen containing fertilizer.
11. The method according to claim 7 , wherein the plant is wheat, maize, rice, or barley.
12. A process for increasing nitrogen use efficiency, biomass, seed yield, increased tillering or a combination thereof, in a monocot plant comprising:
applying an herbicide, insecticide, fertilizer, or combination thereof, to a monocot seed comprising the genetic construct according to claim 5 , and
growing a monocot plant from the seed, wherein the monocot plant has increased nitrogen use efficiency, increased biomass, increased seed yield, increased tillering or a combination thereof compared to a wild-type monocot plant grown under identical conditions.
13. The method according to claim 8 , wherein the plant is wheat, maize, rice, or barley.