Multilayered magnetic micelle compositions and methods for their use
Provided herein are compositions comprising a micelle having a hydrophobic superparmagnetic iron oxide nanoparticle (SPION) core, a first coating comprising a cationic polymer, and a second coating comprising a polynucleotide. Also provided are methods of using the compositions for transfection and/or transformation of a cell with the polynucleotide. Further provided are methods of detecting transfection of a cell with the polynucleotide.
1. A composition comprising a micelle having a hydrophobic superparamagnetic iron oxide nanoparticle (SPION) core, a first coating comprising a cationic polymer, and a second coating comprising a polynucleotide wherein the first coating comprises chitosan and polyethyleneimine (PEI), and wherein chitosan has a chemical formula of I:
wherein “m” is between 1 and 10,000; and wherein PEI has a chemical formula of II:
wherein “m” is between 1 and 10,000, and “n” is between 1 and 10,000.
2. The composition of claim 1 , wherein the micelle comprises multiple copolymers having the chemical formula of:
wherein “m” is between 1 and 10,000, “n” is between 1 and 10,000.
3. The composition of claim 2 , wherein the SPION core comprises a coating of oleic acid and oleylamine.
4. The composition of claim 3 , wherein the SPION core is prepared using iron, 1,2-dodecanediol, oleic acid, oleylamine, and benzyl ether.
5. The composition of claim 2 , wherein a molar weight ratio of chitosan and polynucleotide is between approximately 3:1 and 7:1.
6. The composition of claim 5 , wherein the polynucleotide is at a concentration between approximately 1 and 3 μg/ml.
7. The composition of claim 2 , wherein a molar weight ratio of PEI and polynucleotide is between approximately 7:1 and 3:1.
8. The composition of claim 7 , wherein the polynucleotide is at a concentration between approximately 1 and 3 μg/ml.
9. The composition of claim 2 , wherein a molar weight ratio of chitosan and PEI is between approximately 3:7 and 7:3.
10. The composition of claim 9 , wherein a molar weight ratio of chitosan and PEI is approximately 5:5.
11. The composition of claim 10 , wherein a molar weight ratio of chitosan, PEI and polynucleotide is approximately 5:5:1.
12. The composition of claim 11 , wherein the polynucleotide is at a concentration between approximately 1 and 3 μg/ml.
13. The composition of claim 1 , further comprising a ligand.
14. The composition of claim 13 , wherein in the ligand is an antibody.
15. The composition of claim 14 , wherein the antibody is specific for a prostate stem cell antigen.
16. A method of transfecting a cell with a polynucleotide comprising, contacting the cell with a composition of claim 1 .
17. The method of claim 16 , wherein the micelle comprises multiple copolymers having the chemical formula of III:
wherein “m” is between 1 and 10,000, “n” is between 1 and 10,000.
18. The method of claim 16 , further comprising placing a magnet proximal to the cell.
19. The method of claim 16 , further comprising transforming the cell.
20. The method of claim 17 , wherein a molar weight ratio of chitosan and PEI is approximately 5:5.
21. The method of claim 20 , wherein a molar weight ratio of chitosan, PEI and polynucleotide is approximately 5:5:1.
22. The method of claim 21 , wherein the polynucleotide is at a concentration between approximately 1 and 3 μg/ml.
23. The method of claim 16 , wherein the cell is in vivo.
24. The method of claim 23 , wherein the composition further comprises a cancer cell ligand and the cell is a cancer cell.
25. The method of claim 24 , wherein the cancer cell ligand is an antibody specific for prostate stem cell antigen.
26. The method of claim 16 , further comprising the step of detecting transfection of the cell using magnetic resonance imaging.
27. The method of claim 23 , further comprising the step of detecting transfection of the cell using magnetic resonance imaging.
28. A composition comprising a micelle having a hydrophobic superparamagnetic iron oxide nanoparticle (SPION) core, a first coating comprising a cationic polymer, and a second coating comprising a polynucleotide, wherein the first coating comprises polyethyleneimine and chitosan, wherein the chitosan has a chemical formula of:
wherein “m” is between 1 and 10,000;
and wherein PEI has a chemical formula of:
wherein “m” is between 1 and 10,000, and “n” is between 1 and 10,000,
wherein the micelle further comprises multiple co-polymers having the chemical formula of:
wherein “m” is between 1 and 10,000, “n” is between 1 and 10,000,
wherein the molar weight ratio of chitosan and polynucleotide is between approximately 3:1 and 7:1, the molar weight ratio of PEI and polynucleotide is between approximately 7:1 and 3:1, and the molar weight ratio of PEI to chitosan is between approximately 3:7 and 7:3.
29. The composition of claim 28 , wherein a molar weight ratio of chitosan, PEI and polynucleotide is approximately 5:5:1.
30. The composition of claim 28 , wherein the polynucleotide is at a concentration between approximately 1 and 3 μg/ml.