Polypeptide solution, artificial polypeptide fiber production method and polypeptide purification method using same
A polypeptide solution of the present invention is a polypeptide solution in which a polypeptide derived from natural spider silk proteins is dissolved in a solvent. The solvent contains at least one selected from the following (i)-(iii): (i) DMSO; (ii) DMSO with an inorganic salt; and (iii) DMF with an inorganic salt. Further, in the present invention, an artificial polypeptide fiber is obtained by: using the polypeptide solution as a dope solution; and extruding the dope solution from a spinneret into a desolvation bath so as to eliminate the solvent from the dope solution and form a fiber to produce an undrawn yarn. Moreover, in the present invention, a polypeptide is purified by subjecting the polypeptide solution to heat treatment and thereafter removing an undissolved substance therefrom. Thus, the present invention provides the polypeptide solution whose solute has high solubility and solvent itself is low cost, and that allows dissolution at high temperatures and has high safety: a method for producing an artificial polypeptide fiber: and a method for purifying a polypeptide.
1. A polypeptide solution in which a polypeptide derived from natural spider silk proteins is dissolved in a solvent,
wherein the solvent contains at least one selected from the following (i)-(iii):
(i) Dimethyl sulfoxide;
(ii) Dimethyl sulfoxide with an inorganic salt; and
(iii) N, N-dimethylformamide with an inorganic salt.
2. The polypeptide solution according to claim 1 , wherein, when the polypeptide solution is 100 mass %, the percentage of the polypeptide is in a range of 3 to 45 mass %.
3. The polypeptide solution according to claim 1 , wherein, when the solvent is 100 mass %, the percentage of the inorganic salt is in a range of 0.1 to 20 mass %.
4. The polypeptide solution according to claim 1 , wherein, when the solvent is 100 mass %, the percentage of the at least one substance selected from (i)-(iii) above is 22 mass % or more and 100 mass % or less, and a remainder contains alcohol.
5. The polypeptide solution according to claim 1 , wherein, when the solvent is 100 mass %, the percentage of the at least one substance selected from (i)-(iii) above is 10 mass % or more and 100 mass % or less, and a remainder contains water.
6. The polypeptide solution according to claim 1 , wherein the inorganic salt is at least one selected from alkali metal halides, alkaline-earth metal halides, alkaline-earth metal nitrate, and thiocyanate.
7. The polypeptide solution according to claim 1 , wherein the polypeptide solution is a dope solution.
8. A method for producing an artificial polypeptide fiber using the polypeptide solution according to claim 1 , comprising:
using the polypeptide solution as a dope solution; and
extruding the dope solution from a spinneret into a coagulation liquid in a desolvation bath so as to eliminate a solvent from the dope solution and form a fiber to prepare an undrawn yarn, thereby obtaining an artificial polypeptide fiber.
9. The method for producing an artificial polypeptide fiber according to claim 8 , further comprising:
drawing the undrawn yarn.
10. The method for producing an artificial polypeptide fiber according to claim 8 ,
wherein, when the polypeptide solution is 100 mass %, the percentage of the polypeptide is in a range of 3 to 45 mass %.
11. The method for producing an artificial polypeptide fiber according to claim 8 , wherein, when the solvent is 100 mass %, the percentage of the inorganic salt is in a range of 0.1 to 20 mass %.
12. A method for purifying a polypeptide using the polypeptide solution according to claim 1 , comprising:
subjecting the polypeptide solution to heat treatment and thereafter removing an undissolved substance therefrom.
13. The method for purifying a polypeptide according to claim 12 , wherein the polypeptide derived from natural spider silk proteins is insoluble.
14. The method for purifying a polypeptide according to claim 12 , wherein the heat treatment is performed at 45 to 100° C.
15. The method for purifying a polypeptide according to claim 12 , wherein the undissolved substance is removed by separation through filtration or centrifugation.
16. The method for purifying a polypeptide according to claim 12 , wherein, when the polypeptide solution is 100 mass %, the percentage of the polypeptide is in a range of 3 to 45 mass %.
17. The method for purifying a polypeptide according to claim 12 , wherein, when the solvent is 100 mass %, the percentage of the inorganic salt is in a range of 0.1 to 20 mass %.