DNA sample preparation and sequencing
This disclosure describes, in one aspect, a method for preparing DNA molecule for sequencing. Generally, the method includes fragmenting the DNA molecule into double-stranded fragments; amplifying at least a portion of the double-stranded fragments; circularizing the fragments so that the first end of the fragment comprises a first loop connecting the strands and the second end of the fragment comprises a second loop connecting the strands; annealing a first sequencing primer to the first loop oriented to sequence at least a portion of one strand of the fragment; and annealing a second sequencing primer to the second loop oriented to sequence at least a portion of the other strand of the fragment. In another aspect, this disclosure describes a method for sequencing a DNA molecule. Generally, the method includes fragmenting the DNA molecule into double-stranded fragments; amplifying at least a portion of the double-stranded fragments; circularizing the fragments so that the first end of the fragment comprises a first loop connecting the strands and the second end of the fragment comprises a second loop connecting the strands; and sequencing at least one of the DNA strands.
1. A method for preparing a DNA molecule for sequencing, the method comprising:
fragmenting the DNA molecule into double-stranded fragments;
amplifying at least a portion of the double-stranded fragments;
preparing an emulsion that comprises a first subset of droplets and a second subset of droplets, wherein:
the first subset of droplets comprises amplified DNA fragments; and
the second subset of droplets comprises a plurality of transposons selected to insert into the amplified DNA fragments and comprising loops that, when the transposon inserts into a fragment, circularizes the fragment;
circularizing the fragments so that the first end of a fragment comprises a first loop connecting the strands of the fragment and the second end of the fragment comprises a second loop connecting the strands of the fragment;
annealing a first sequencing primer to the first loop oriented to sequence at least a portion of one strand of at least one fragment; and
annealing a second sequencing primer to the second loop oriented to sequence at least a portion of the other strand of the fragment.
2. A method for sequencing a DNA molecule, the method comprising:
fragmenting the DNA molecule into double-stranded fragments;
amplifying at least a portion of the double-stranded fragments;
preparing an emulsion that comprises a first subset of droplets and a second subset of droplets, wherein:
the first subset of droplets comprises amplified DNA fragments; and
the second subset of droplets comprises a plurality of transposons selected to insert into the amplified DNA fragments and comprising loops that, when the transposon inserts into a fragment, circularizes the fragment;
circularizing the fragments so that the first end of a fragment comprises a first loop connecting the strands of the fragment and the second end of the fragment comprises a second loop connecting the strands of the fragment; and
sequencing at least one of the DNA strands.
3. The method of claim 2 wherein sequencing at least one of the strands comprises sequencing-by-synthesis or sequencing-by-ligation.
4. The method of claim 3 wherein sequencing at least one of the DNA strands comprises:
annealing a first sequencing primer to the first loop oriented to sequence at least a portion of one strand of the fragment;
annealing a second sequencing primer to the second loop oriented to sequence at least a portion of the other strand of the fragment; and
performing sequencing-by-synthesis.
5. The method of claim 2 wherein:
sequencing at least one of the DNA strands comprises generating a read; and
the method further comprises assembling a plurality of reads to generate the sequence of the DNA molecule.
6. The method of claim 2 further comprising preparing an emulsion comprising a plurality of droplets that contain the circularized DNA fragments.
7. The method of claim 2 wherein at least a portion of the DNA fragments are amplified prior to being circularized.
8. The method of claim 2 wherein droplets in the first subset of droplets comprise an electrical charge and droplets in the second subset of droplets comprise an electrical charge opposite of the electrical charge of the droplets in the first subset of droplets.
9. The method of claim 8 wherein circularizing the fragments comprises merging at least one droplet from the first subset of droplets and at least one droplet from the second subset of droplets.
10. The method of claim 2 further comprising immobilizing at least a portion of the circularized DNA fragments to a substrate.
11. The method of claim 10 wherein the substrate comprises a multi-well array.
12. The method of claim 11 further comprising detecting light signals that correspond to nucleotide bases in the DNA sequence.
13. The method of claim 10 wherein the substrate comprises a DG-ISFET device.
14. The method of claim 13 further comprising detecting transient pH readings that correspond to nucleotide bases in the DNA sequence.
15. The method of claim 2 wherein fragmenting the DNA molecule comprises shearing the DNA molecule.
16. The method of claim 2 wherein fragmenting the DNA molecule and circularizing the fragments are performed in a single step that comprises inserting a plurality of transposons into the DNA molecule, wherein transposons insert loops onto the ends of DNA fragments that are generated by inserting the plurality of transposons into the DNA molecule, thereby circularizing the fragments.
17. The method of claim 1 further comprising preparing an emulsion comprising a plurality of droplets that contain the circularized DNA fragments.
18. The method of claim 1 wherein at least a portion of the DNA fragments are amplified prior to being circularized.
19. The method of claim 1 wherein droplets in the first subset of droplets comprise an electrical charge and droplets in the second subset of droplets comprise an electrical charge opposite of the electrical charge of the droplets in the first subset of droplets.
20. The method of claim 19 wherein circularizing the fragments comprises merging at least one droplet from the first subset of droplets and at least one droplet from the second subset of droplets.
21. The method of claim 1 further comprising immobilizing at least a portion of the circularized DNA fragments to a substrate.
22. The method of claim 21 wherein the substrate comprises a multi-well array.
23. The method of claim 22 further comprising detecting light signals that correspond to nucleotide bases in the DNA sequence.
24. The method of claim 21 wherein the substrate comprises a DG-ISFET device.
25. The method of claim 13 further comprising detecting transient pH readings that correspond to nucleotide bases in the DNA sequence.
26. The method of claim 1 wherein fragmenting the DNA molecule comprises shearing the DNA molecule.
27. The method of claim 1 wherein fragmenting the DNA molecule and circularizing the fragments are performed in a single step that comprises inserting a plurality of transposons into the DNA molecule, wherein transposons insert loops onto the ends of DNA fragments that are generated by inserting the plurality of transposons into the DNA molecule, thereby circularizing the fragments.