Polypeptides having endoglucanase activity and polynucleotides encoding same
Provided are isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. A method for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material with an enzyme composition in the presence of the polypeptide having endoglucanase activity;
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation,
wherein the polypeptide having endoglucanase activity is selected from:
a polypeptide having at least 98% sequence identity to amino acids 18 to 414 of SEQ ID NO: 2:
a polypeptide encoded by a polynucleotide having at least 98% sequence identity to nucleotides 52 to 1242 of SEQ ID NO: 1.
2. A method of fermenting a cellulosic material, comprising: fermenting the cellulosic material with one or more fermenting microorganisms, wherein the cellulosic material is saccharified with an enzyme composition in the presence of the polypeptide having endoglucanase activity,
wherein the polypeptide having endoglucanase activity is selected from:
a polypeptide having at least 98% sequence identity to amino acids 18 to 414 of SEQ ID NO: 2:
a polypeptide encoded by a polynucleotide having at least 98% sequence identity to nucleotides 52 to 1242 of SEQ ID NO: 1.
3. The method of claim 2 , wherein the fermenting of the cellulosic material produces a fermentation product.
4. The method of claim 3 , further comprising recovering the fermentation product from the fermentation.
5. The method of claim 1 , wherein the polypeptide having endoglucanase activity has at least 98% sequence identity to amino acids 18 to 414 of SEQ ID NO: 2.
6. The method of claim 1 , wherein the polypeptide having endoglucanase activity has at least 99% sequence identity to amino acids 18 to 414 of SEQ ID NO: 2.
7. The method of claim 1 , wherein the polypeptide having endoglucanase activity comprises amino acids 18 to 414 of SEQ ID NO: 2.
8. The method of claim 1 , wherein the polypeptide having endoglucanase activity is a variant of amino acids 18 to 414 of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions.
9. The method of claim 1 , wherein the cellulosic material is pretreated before saccharification.
10. The method of claim 2 , wherein the polypeptide having endoglucanase activity has at least 98% sequence identity to amino acids 18 to 414 of SEQ ID NO: 2.
11. The method of claim 2 , wherein the polypeptide having endoglucanase activity has at least 99% sequence identity to amino acids 18 to 414 of SEQ ID NO: 2.
12. The method of claim 2 , wherein the polypeptide having endoglucanase activity comprises amino acids 18 to 414 of SEQ ID NO: 2.
13. The method of claim 2 , wherein the polypeptide having endoglucanase activity is a variant of amino acids 18 to 414 of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions.
14. The method of claim 2 , wherein the cellulosic material is pretreated before saccharification.