PLATELET LYSATE GEL
The invention concerns a pharmaceutical composition comprising a platelet lysate and its use to treat a wound, an anal fissure, vaginal atrophy or a wrinkle.
1 - 11 . (canceled)
12 . A method of producing a platelet lysate comprising subjecting a population of platelets to at least one freeze-thaw cycle, wherein the freeze portion of each cycle is carried out at a temperature lower than or equal to −78° C.
13 . A method according to claim 12 , wherein the method comprises subjecting the population of platelets to three or four freeze-thaw cycles.
14 . A method according to claim 12 , wherein liquid nitrogen is used as a cryogenic means whereby immersion in the freeze portion of each cycle results in 95% or more lysis of platelets resulting in greater growth factor release and improved function in cell growth, repair and regeneration measurable by but not limited to fibroblast and PGDF assay.
15 . A method according to claim 12 , where dry ice (solid carbon dioxide) and an organic solvent are used as a cryogenic means in the freeze portion of each cycle.
16 . A method of producing platelet lysate comprising subjecting a population of platelets to mechanical homogenisation (‘waring blender’), liquid homogenization, sonication, freeze-thaw using alcohol and dry ice or other means, mechanical grinding in liquid nitrogen at >75%, but typically >90%, lysis to release growth factors from platelets and alpha granules into solution whereas rotating blades grind and disperse cells and tissues; cell or tissue suspensions are sheared by forcing them through a narrow space; high frequency sound waves shear cells; repeated cycles of freezing and thawing disrupt cells through ice crystal formation; grinding tissue, frozen in liquid nitrogen wherein lysis releases growth factors from platelets and alpha granules into solution.
17 . A method according to claim 12 or 16 , wherein the method further comprises lyophilising the platelet lysate.
18 . A platelet lysate produced using a method according to claim 12 or 16 .
19 . A platelet lysate according to claim 18 for use in treating a wound, an anal fissure, vaginal atrophy or a wrinkle in a patient in need thereof.
20 . A method of treating a wound, an anal fissure, vaginal atrophy or a wrinkle in a patient in need thereof, comprising administering to the patient a therapeutically effective amount of a platelet lysate according to claim 18 .
21 - 22 . (canceled)