Medium composition for rejuvenating stem cells
The present invention relates to a medium composition for transforming stem cells from an aged person into young stem cells, and more particularly to a medium composition for culturing stem cells, which is used to rejuvenate stem cells from an aged person so as to have characteristics similar to those of the stem cells of young people, and to a method for rejuvenating stem cells, which comprises culturing stem cells from an aged person in the medium composition. According to the present invention, even mesenchymal stem cells collected from over 60 years old patients can be transformed into young mesenchymal stem cells having high differentiation ability, high telomerase activity, and high ability to express stem cell markers. Thus, the present invention can significantly increase the efficacy of cell therapy employing mesenchymal stem cells.
1. A method for rejuvenating mesenchymal stem cells from an aged person, the method comprising:
culturing mesenchymal stem cells from an aged person in a medium composition containing FBS (fetal bovine serum), 0.5-1 ng/ml selenium, NAC (N-acetyl-L-cysteine), 10-50 ng/ml EGF (epidermal growth factor), 1-100 ng/ml bFGF (basic fibroblast growth factor), insulin or insulin-like factor, and hydrocortisone,
wherein the aged person is a 60-120 year old person,
wherein said rejuvenating is one or more of the following characteristics:
(i) promoting proliferation rate;
(ii) increasing of telomerase activity;
(iii) increasing of pluripotency markers expression level; and
(iv) increasing of differentiation ability.