Graphene-biomolecule bioelectronic devices
Provided are devices and methods featuring a nanoelectronic interface between graphene devices (for example, field effect transistors or FETs) and biomolecules such as proteins, which in turn provides a pathway for production of bioelectronic devices that combine functionalities of the biomolecular and inorganic components. In one exemplary application, one may functionalize graphene FETs with fluorescent proteins to yield hybrids that respond to light at wavelengths defined by the optical absorption spectrum of the protein. The devices may also include graphene in electronic communication with a biomolecule that preferentially binds to a particular analyte.
1. A device, comprising:
a biomolecule comprising a fluorescent protein having an optical absorption peak at around a particular wavelength of excitation illumination,
the biomolecule being in electronic communication with a graphene body so as to define a hybrid body,
the graphene body being in the form of a flat single-atom thick sheet,
the hybrid body having a photocurrent response at the particular wavelength of excitation illumination;
a monitor configured to monitor the photocurrent response of the hybrid body; and
a source of illumination configured to deliver illumination at around the particular wavelength of excitation illumination.
2. The device of claim 1 , wherein the protein comprises a histidine tag.
3. The device of claim 1 , wherein the protein is bound to the graphene body by a peptide sequence.
4. The device of claim 1 , wherein the protein comprises a reactive amino acid.
5. The device of claim 1 , wherein the graphene comprises Diimide-activated amidation.
6. The device of claim 1 , further comprising a cysteine-graphene linkage.
7. The device of claim 1 , whether comprising an amide bond between the protein and graphene, an imine bond between the protein and graphene, a thiourea bond between the protein and graphene, an aminoalcohol bond between the protein and graphene.
8. The device of claim 1 , wherein the fluorescent protein comprises a green fluorescent protein, a yellow fluorescent protein, a red fluorescent protein, a cyan fluorescent protein, or any combination thereof.
9. The device of claim 1 , wherein the fluorescent protein comprises a fluorescent redox cofactor.
10. The device of claim 1 , further comprising a flavoprotein, a heme containing protein wherein an iron has been substituted by zinc, or any combination thereof.
11. The device of claim 1 , wherein the fluorescent protein comprises tryptophan.
12. The device of claim 1 , wherein the electronic communication between the biomolecule and the graphene body includes an interaction between a nickel-nitriloacetic acid group and a histidine residue.
13. The device of claim 1 , wherein the particular wavelength of excitation illumination is a wavelength between about 1 nm and about 900 nm.
14. A device, comprising:
a graphene body in the form of a flat single-atom thick sheet in electronic communication with a biomolecule that preferentially binds to a binding partner, thereby forming a hybrid body,
the biomolecule comprising a green fluorescent protein, a yellow fluorescent protein, a red fluorescent protein, a cyan fluorescent protein, or any combination thereof,
the hybrid body exhibiting a measureable photocurrent response when the biomolecule binds to the binding partner.
15. The device of claim 1 , further comprising a plurality of biomolecules having different absorption peaks in electronic communication with the graphene body.
16. The device of claim 14 , wherein further comprising a plurality of biomolecules having different binding partners in electronic communication with the graphene body.
17. The device of claim 1 , further comprising a fusion protein.
18. The device of claim 1 , further comprising a chitin binding protein, a maltose binding protein, glutathione-S-transferase, an epitope tag, or any combination thereof.