Hybrid polypeptides having cellobiohydrolase activity and polynucleotides encoding same
The present invention provides hybrid polypeptides having cellobiohydrolase activity. The present invention also provides polynucleotides encoding the hybrid polypeptides; nucleic acid constructs, vectors and host cells comprising the polynucleotides; and processes of using the hybrid polypeptides.
1. A hybrid polypeptide having cellobiohydrolase activity, comprising:
(a) a first polypeptide fragment which comprises a cellobiohydrolase catalytic domain, wherein the cellobiohydrolase catalytic domain has at least 96% sequence identity to amino acids 19 to 455 of SEQ ID NO: 2 or is a fragment of amino acids 19 to 455 of SEQ ID NO: 2 that has a cellobiohydrolase activity; and
(b) a second polypeptide fragment which comprises a carbohydrate binding domain, wherein the carbohydrate binding domain has at least 96% sequence identity to amino acids 468 to 532 of SEQ ID NO: 4 or is a fragment of amino acids 468 to 532 of SEQ ID NO: 4 that has carbohydrate binding activity;
wherein the catalytic domain is at the N-terminal end of the hybrid polypeptide and the carbohydrate binding domain is at the C-terminal end of the catalytic domain.
2. The hybrid polypeptide of claim 1 , wherein the first polypeptide fragment has at least 96% sequence identity to amino acids 19 to 455 of SEQ ID NO: 2.
3. The hybrid polypeptide of claim 1 , wherein the first polypeptide fragment has at least 97% sequence identity to amino acids 19 to 455 of SEQ ID NO: 2.
4. The hybrid polypeptide of claim 1 , wherein the first polypeptide fragment has at least 98% sequence identity to amino acids 19 to 455 of SEQ ID NO: 2.
5. The hybrid polypeptide of claim 1 , wherein the first polypeptide fragment has at least 99% sequence identity to amino acids 19 to 455 of SEQ ID NO: 2.
6. The hybrid polypeptide of claim 1 , wherein the first polypeptide fragment comprises amino acids 19 to 455 of SEQ ID NO: 2.
7. The hybrid polypeptide of claim 1 , wherein the first polypeptide fragment consists of amino acids 19 to 455 of SEQ ID NO: 2.
8. The hybrid polypeptide of claim 1 , wherein the second polypeptide fragment has at least 96% sequence identity to amino acids 468 to 532 of SEQ ID NO: 4.
9. The hybrid polypeptide of claim 1 , wherein the second polypeptide fragment has at least 97% sequence identity to amino acids 468 to 532 of SEQ ID NO: 4.
10. The hybrid polypeptide of claim 1 , wherein the second polypeptide fragment has at least 98% sequence identity to amino acids 468 to 532 of SEQ ID NO: 4.
11. The hybrid polypeptide of claim 1 , wherein the second polypeptide fragment has at least 99% sequence identity to amino acids 468 to 532 of SEQ ID NO: 4.
12. The hybrid polypeptide of claim 1 , wherein the second polypeptide fragment comprises amino acids 468 to 532 of SEQ ID NO: 4.
13. The hybrid polypeptide of claim 1 , wherein the second polypeptide fragment consists of amino acids 468 to 532 of SEQ ID NO: 4.
14. The hybrid polypeptide of claim 7 , wherein the second polypeptide fragment consists of amino acids 468 to 532 of SEQ ID NO: 4.
15. A polynucleotide encoding the hybrid polypeptide of claim 1 .
16. A host cell comprising the polynucleotide of claim 15 , wherein the polynucleotide is operably linked to control sequences that provide for the expression of the hybrid polypeptide by the host cell.
17. A method of producing a hybrid polypeptide having cellobiohydrolase activity, comprising:
(a) cultivating the host cell of claim 16 under conditions suitable for the expression of the hybrid polypeptide; and
(b) recovering the hybrid polypeptide.
18. A process for degrading or converting a cellulosic material, comprising: treating the cellulosic material with a hybrid polypeptide of claim 1 , an endoglucanase, a beta-glucosidase, and a GH61 polypeptide.
19. The process of claim 18 , wherein the cellulosic material is pretreated.
20. The process of claim 18 , further comprising recovering the degraded cellulosic material.
21. The process of claim 20 , wherein the degraded cellulosic material is a sugar.
22. The process of claim 21 , wherein the sugar is selected from the group consisting of arabinose, galactose, glucose, mannose, and xylose.
23. A process for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material with a hybrid polypeptide of claim 1 , an endoglucanase, a beta-glucosidase, and a GH61 polypeptide;
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product.
24. The process of claim 23 , wherein the cellulosic material is pretreated.
25. The process of claim 23 , wherein steps (a) and (b) are performed simultaneously in a simultaneous saccharification and fermentation.
26. The process of claim 23 , wherein the fermentation product is an alcohol, an alkane, a cycloalkane, an alkene, an amino acid, a gas, isoprene, a ketone, an organic acid, or polyketide.