Method for producing natural killer cells, natural killer cells produced thereby, and composition for treating cancers and infectious diseases containing the same
The present invention relates to a method for producing natural killer cells (hereinafter, referred to as “NK cells”), NK cells produced thereby, and a composition for treating cancers and infectious diseases containing the same. The present invention provides a method for producing NK cells, which maintain high cytotoxicity and cell viability to exhibit high therapeutic effects against cancers and infectious diseases and can be cultured ex vivo at high efficiency and high concentration. In addition, a culture method which maintains cell concentration at a constant level is used for the production of NK cells, and thus the overgrowth of the cells can be prevented so that the cells can be maintained at an optimal state. Particularly, even when the cells are thawed after freezing, the function of the cells is not impaired, and the NK cells can maintain high cell viability and cytotoxicity. Thus, the NK cells can be easily stored and supplied in a liquid or frozen state without needing an additional treatment process.
1. A method for producing NK cells, comprising the steps of: (i) isolating peripheral blood leukocytes and NK cells from human peripheral blood; (ii) carrying out stationary culture of NK cells for 2-15 days in a medium containing anti-CD3 antibody, cytokine and inactivated peripheral blood mononuclear cells (PBMCs) as feeder cells to stimulate by cell-cell contact; (iii) after completion of stationary culture in step (ii), re-stimulating the cells by addition of cytokine, anti-CD3 antibody and inactivated peripheral blood mononuclear cells (PBMCs) as feeder cells, (iv) carrying out stationary culture for 2-7 days to stimulate cause cell-cell contact; (v) after completion of the stationary culture, adding to the cells a medium containing cytokine required for stationary or suspension culture, and carrying out stationary or suspension culture while maintaining cell concentration and cytokine concentration at constant levels.
2. The method of claim 1 , wherein step (iii) of re-stimulating the cells and carrying out stationary culture is repeated twice or more.
3. The method of claim 1 , wherein the suspension culture in step (v) is performed by using a reactor selected from the group consisting of a shaking flask, a shaking incubator, a fermentor, a T-flask, and a disposable cell culture bag.
4. The method of claim 1 , wherein the stationary culture in step (ii) or (iii) and the stationary or suspension culture in step (v) are performed in a same or different reactor.
5. The method of claim 1 , the concentrations of cell and cytokine in the medium are measured during the stationary or suspension culture in step (v), and the medium containing cytokine are added in order to maintain cell and cytokine concentration at constant levels.
6. The method of claim 1 , wherein the anti-CD3 antibody is one or more selected from the group consisting of OKT3, UCHT1, and HIT3a.
7. The method of claim 6 , wherein the anti-CD3 antibody is OKT-3 antibody.
8. The method of claim 1 , wherein the cytokine is one or more selected from the group consisting of interleukin-2 (IL-2), interleukin-12 (IL-12), interleukin-15 (IL-15), interleukin-18 (IL-18), and interleukin-21 (IL-21).
9. The method of claim 8 , wherein the cytokine is IL-2.