IP Library Granted Patent US 9,291,557
Granted Patent B2
US 9,291,557 · App. 14/368,499 · Granted Mar 22, 2016

Localized surface plasmon resonance mercury detection system and methods

Inventors: Jay James (Berkeley, CA); Donald Lucas (Moraga, CA); Jeffrey Scott Crosby (Berkeley, CA); Catherine P. Koshland (Atherton, CA)
Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
G01N21/554G01J3/42G01N33/0045G01J2003/425G01N2201/088
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Quick Facts
Patent No.
US 9,291,557
App. No.
14/368,499
Granted
Mar 22, 2016
Kind
B2
Abstract

A mercury detection system that includes a flow cell having a mercury sensor, a light source and a light detector is provided. The mercury sensor includes a transparent substrate and a submonolayer of mercury absorbing nanoparticles, e.g., gold nanoparticles, on a surface of the substrate. Methods of determining whether mercury is present in a sample using the mercury sensors are also provided. The subject mercury detection systems and methods find use in a variety of different applications, including mercury detecting applications.

Claims (41)

1. A mercury detection system comprising:

a flow cell comprising a mercury sensor, wherein the mercury sensor comprises:

a transparent substrate; and

a submonolayer of spherical gold nanoparticles on a surface of the substrate, wherein the spherical gold nanoparticles are substantially free of a surface coating;

a light source that directs light from the light source to the mercury sensor;

a light detector that detects a localized surface plasmon resonance (LSPR) signal from the spherical gold nanoparticles; and

a heat source that heats the mercury sensor to regenerate the mercury sensor,

wherein the system determines whether mercury is present in a sample based on a rate of change of the detected LSPR signal from the spherical gold nanoparticles.

2. The mercury detection system of claim 1 , wherein the submonolayer has a density of 5×10 12 gold nanoparticles/cm 2 or less.

3. The mercury detection system of claim 1 , wherein the gold nanoparticles have a surface area to volume ratio of 0.2 or more.

4. The mercury detection system of claim 1 , further comprising a gas source in communication with the flow cell and configured to provide a flow of a gas through the flow cell.

5. The mercury detection system of claim 1 , wherein the light source comprises a visible light source.

6. The mercury detection system of claim 1 , wherein the light detector comprises a UV-Vis photodetector.

7. The mercury detection system of claim 1 , wherein the system is configured to detect mercury vapor at a concentration of 100 μg/m 3 or less.

8. The mercury detection system of claim 1 , wherein the heat source regenerates the mercury sensor without a significant decrease in sensitivity of the mercury sensor.

9. The mercury detection system of claim 1 , wherein the sample is a gaseous sample.

10. The mercury detection system of claim 9 , wherein the flow cell accommodates a flow rate of the gaseous sample of 0.01 L/min to 20 L/min.

11. A method for determining whether mercury is present in a sample, the method comprising:

contacting a sample to a mercury sensor comprising:

a transparent substrate; and

a submonolayer of spherical gold nanoparticles on a surface of the substrate, wherein the spherical gold nanoparticles are substantially free of a surface coating;

directing light from a light source to the sample-contacted mercury sensor;

detecting a localized surface plasmon resonance (LSPR) signal from the spherical gold nanoparticles;

determining whether mercury is present in the sample based on a rate of change in the detected LSPR signal; and

regenerating the mercury sensor by heating the mercury sensor.

12. The method of claim 11 , wherein the contacting comprises flowing a gaseous sample through a flow cell comprising the mercury sensor.

13. The method of claim 12 , wherein the gaseous sample has a flow rate of 0.01 L/min to 20 L/min.

14. The method of claim 11 , further comprising quantifying the amount of mercury in the sample.

15. The method of claim 14 , wherein the quantifying comprises determining the amount of mercury in the sample based on the rate of change in the localized surface plasmon resonance wavelength of the gold nanoparticles.

16. The method of claim 14 , wherein the quantifying comprises determining the amount of mercury in the sample based on the rate of change of the absorbance (A) at wavelength (λ) bands of greatest slope (dA/dλ) near the localized surface plasmon resonant peak.

17. The method of claim 11 , wherein the contacting comprises flowing a gaseous sample through a nozzle perpendicular to the mercury sensor.

18. The method of claim 11 , further comprising contacting the sensor with water vapor before contacting the sample to the sensor.

19. The method of claim 11 , wherein the regenerating does not significantly decrease sensitivity of the mercury sensor.

20. A system for determining whether mercury is present in a sample, the system comprising:

a nozzle for directing flow of a fluid onto a mercury sensor, wherein the mercury sensor comprises:

a transparent substrate; and

a submonolayer of spherical gold nanoparticles on a surface of the substrate, wherein the spherical gold nanoparticles are substantially free of a surface coating;

a light source that directs light from the light source to the mercury sensor;

a light detector that detects a localized surface plasmon resonance (LSPR) signal from the spherical gold nanoparticles; and

a heat source that heats the mercury sensor to regenerate the mercury sensor,

wherein the system determines whether mercury is present in a sample based on a rate of change of the detected LSPR signal from the spherical gold nanoparticles.

Assignments (2)
CONFIRMATORY LICENSE Recorded Jun 15, 2016
From: UNIVERSITY OF CALIFORNIA BERKELEY
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 039014/0098 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 8, 2014
From: JAMES, JAY; LUCAS, DONALD; CROSBY, JEFFREY SCOTT; KOSHLAND, CATHERINE P.
To: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Reel/Frame 033912/0182 →
Continuity (3)
Provisional Application 61585542 · Jan 11, 2012
Provisional Application 61587546 · Jan 17, 2012
Related Publication 20140333933A1 · Nov 13, 2014