Methods for increasing the infectivity of viruses utilizing alkyne-modified fatty acids
View Patent ↗Methods of using viruses labeled with alkyne-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant, an insect or an animal infected with a virus or to increase the infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a virus, such as human immunodeficiency virus, with an alkyne-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. The viruses labeled with alkyne-modified biomolecules may be combined with a pharmaceutically acceptable excipient to produce a pharmaceutical composition, optionally containing another anti-viral agent and/or a delivery agent, such as a liposome.
1. A method of enhancing the infectivity of a human immunodeficiency virus, the method comprising contacting a cell infected with the human immunodeficiency virus with an alkyne-modified fatty acid or physiologically acceptable salt thereof in an amount effective to label the human immunodeficiency virus with the alkyne-modified fatty acid or physiologically acceptable salt thereof and thereby enhance the infectivity of the labeled human immunodeficiency virus, wherein the alkyne-modified fatty acid or physiologically acceptable salt thereof has the formula:
Y—CH 2 —X—CO 2 H
wherein,
Y is an ethynyl group; and
X is a linear carbon chain comprising 8 to 15 carbons; and wherein the alkyne-modified fatty acid is one that is attached directly or indirectly to a protein through an intracellular myristoylation or palmitoylation pathway.
2. The method of claim 1 , wherein the cell is a human cell or a non-human animal cell.
3. The method of claim 1 , wherein the human immunodeficiency virus is HIV-1.
4. The method of claim 1 , wherein the alkyne-modified fatty acid is 15-ethynylpentadecanoic acid, 12-ethynyldodecanoic acid, or physiologically acceptable salt thereof.
5. The method of claim 1 , wherein the alkyne-modified fatty acid is
or physiologically acceptable salt thereof.
6. The method of claim 1 , wherein the contacting is performed in a solution comprising at least one of animal serum, amino acids, buffers, fatty acids, glucose, hormones, inorganic salts, lipids, metal ion chelators, peptides, surfactants, trace metals, and vitamins.
7. The method of claim 1 , wherein the alkyne-modified fatty acid is one that is attached directly or indirectly to a protein through an intracellular myristoylation pathway.
8. The method of claim 1 , wherein the alkyne-modified fatty acid is one that is attached directly or indirectly to a protein through an intracellular palmitoylation pathway.
9. The method of claim 4 , wherein the alkyne-modified fatty acid is 15-ethynylpentadecanoic acid or physiologically acceptable salt thereof.