Method for quantifying cholesterol in high density lipoprotein 3

1. A method for quantifying cholesterol in high-density lipoprotein 3 in a test sample from a human patient, said method comprising:

obtaining the test sample of serum or blood plasma containing high-density lipoprotein 3 from the human patient;

adding a surfactant(s) selected from the group consisting of polyoxyethylene polycyclic phenyl ether and polyoxyethylene styrenated phenyl ether to the test sample or a dilution thereof at a surfactant concentration of 0.01 to 5.0% w/v to selectively solubilize high-density lipoprotein 3 and release the cholesterol therefrom; and

quantifying the cholesterol in the high-density lipoprotein 3 of the test sample by optically measuring the cholesterol released from the selectively solubilized high-density lipoprotein 3,

wherein high-density lipoprotein 2 is not selectively solubilized by the addition of the surfactant(s) and high-density lipoprotein 2 cholesterol is not included in the quantification of the cholesterol.

2. The method of claim 1 , wherein the polyoxyethylene polycyclic phenyl ether is selected from the group consisting of Newcol-610 and Newcol-710.

3. The method of claim 1 , wherein the polyoxyethylene styrenated phenyl ether is selected from the group consisting of ADEKATOL PC-10, BLAUNON DSP-12.5, BLAUNON TSP-16, Noigen EA-137, and Noigen EA-157.

4. The method of claim 1 , wherein the released cholesterol is optically measured via a cholesterol esterase, cholesterol oxidase, and a quinone pigment.

5. The method of claim 1 , wherein the concentration of the surfactant is 0.05 to 3.0% w/v.

6. A method for quantifying cholesterol in high-density lipoprotein 3, comprising:

protecting and/or eliminating cholesterol in lipoproteins other than HDL or HDL3, and allowing a surfactant to selectively solubilize high-density lipoprotein 3 releasing the cholesterol from the high-density lipoprotein 3 into the test sample and quantifying the released cholesterol,

wherein high-density lipoprotein 2 is not selectively solubilized by the addition of the surfactant and high-density lipoprotein 2 cholesterol is not included in the quantified cholesterol,

wherein the surfactant that selectively solubilizes high-density lipoprotein 3 is selected from the group consisting of polyoxyethylene polycyclic phenyl ether and polyoxyethylene styrenated phenyl ether,

wherein the protecting and/or eliminating cholesterol in lipoproteins other than HDL or HDL3 is carried out in the presence of a surfactant that reacts with lipoproteins other than HDL.

7. The method of claim 6 , wherein the surfactant that reacts with lipoproteins other than HDL comprises polyoxyethylene sorbitan derivatives, polyoxyethylene-polyoxypropylene condensates, polyoxyethylene-stearylamine, anionic surfactants, amphoteric surfactants, and cationic surfactants.

8. A method for quantifying cholesterol in high-density lipoprotein 3, said method comprising:

allowing a surfactant(s) which react(s) with high-density lipoprotein 3 to react with a test sample; and

quantifying cholesterol optically, which surfactant(s) is(are) at least one selected from the group consisting of polyoxyethylene polycyclic phenyl ether and polyoxyethylene styrenated phenyl ether,

wherein high-density lipoprotein 2 is not included in the quantified cholesterol.

9. The method of claim 1 , wherein prior to said adding step, cholesterol in lipoproteins other than HDL or HDL3 is protected and/or eliminated.

10. The method of claim 1 , wherein cholesterol is quantified by measuring absorbance of light.

11. The method of claim 7 , wherein the anionic surfactant is an amide ether sulfate or polyoxyethylene alkyl ether sodium sulfate.

12. The method of claim 7 , wherein the amphoteric surfactant is coconut oil fatty acid-amidopropyldimethyl-aminoacetic acid betaine, alkyl dimethyl-aminoacetic acid betaine or lauryl betaine.

13. The method of claim 7 , wherein the amphoteric surfactant cationic surfactant is lauryl trimethyl ammonium chloride.