Isolated polynucleotides expressing or modulating microRNAs or targets of same, transgenic plants comprising same and uses thereof
Isolated polynucleotides expressing or modulating microRNAs or targets of same are provided. Also provided are transgenic plants comprising same and uses thereof in improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant.
1. A method of improving drought or salt tolerance of a soybean plant, the method comprising:
transforming a soybean plant with an exogenous DNA construct comprising a plant-expressible promoter operably linked to the miRNA precursor polynucleotide having the nucleic acid sequence as set forth in SEQ ID NO: 11889 which encodes the mature miRNA sequence as set forth in SEQ ID NO: 33, growing the transformed plant under drought or salt stress conditions, and wherein over-expression of said miRNA precursor polynucleotide encoding said miRNA sequence in said transformed soybean plant improves drought or salt tolerance of the transformed soybean plant as compared to a control soybean plant lacking said exogenous DNA construct and grown under the same growth and said stress conditions.