High-Purity Rubusoside And Process For Producing Of The Same
The invention provides a process of producing Rubusoside from steviol glycosides of Stevia rebaudiana plant. The process is useful for producing high purity Rubusoside with purity greater than 95% (dry basis). High purity rubusoside is useful as in combination with other caloric and non-caloric sweeteners as well as non-caloric sweetener in various food and beverage compositions. The high purity rubusoside is useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectionaries, bakeries, cookies, chewing gums, and alike.
1 . A process for preparing Rubusoside from steviol glycosides of Stevia rebaudiana comprising the steps of:
a. providing initial steviol glycosides of Stevia rebaudiana;
b. dissolving the initial steviol glycosides in the water;
c. providing an enzyme with glycosyl hydrolase activity to obtain reaction mixture;
d. incubating the reaction mixture to facilitate complete or partial transformation of Stevioside to Rubusoside;
e. terminating the reaction by thermal inactivation of enzyme;
f. recovering and purifying high purity Rubusoside from reaction mixture.
2 . A process of claim 1 wherein step (a) the steviol glycoside is Stevia extract, mixture of steviol glycosides or pure Stevioside.
3 . A process of claim 1 wherein step (b) the concentration of dissolved steviol glycosides is 1-50% (wt/vol), preferably 5-30% (wt/vol), more preferably 8-10% (wt/vol).
4 . A process of claim 1 wherein step (c) the enzyme is selected from the group of rhamnosidase, β-glucosidase, hesperidinase, naringinase, pectinase, cellulase, and other enzymes with glycosyl hydrolase activity, in free or immobilized forms.
5 . A process of claim 1 wherein step (d) the molar yield of Rubusoside from Stevioside is 1-100%, preferably 80-100% and more preferably 95-100%.
6 . A process of claim 1 wherein step (e) the reaction termination includes heat treatment, activated carbon treatment.
7 . A process of claim 1 wherein step (f) one or more purification techniques are employed selected from the group of ion exchange treatment, macroporous adsorbent treatment, membrane process, chromatographic separation, and crystallization from various solvent systems.
8 . A process of claim 1 wherein step (f) the recovery and purification of Rubusoside comprises the steps of
a. providing reaction mixture containing Rubusoside;
b. contacting the reaction mixture with macroporous adsorbent resin;
c. eluting the adsorbed Rubusoside with aqueous alcohol;
d. removing the alcohol from eluate and drying the solution to yield a purified Rubusoside.
9 . A process of claim 1 wherein step (f) the recovery and purification of Rubusoside comprises the steps of:
a. providing dried reaction mixture containing Rubusoside;
b. dissolving the reaction mixture in a first aqueous alcoholic solution to obtain a first mixture;
c. inducing crystallization in the first mixture by temperature gradient treatment;
d. filtering the first mixture to obtain a first precipitate and a first filtrate;
e. dissolving the first precipitate in a second aqueous alcoholic solution to obtain a second mixture;
f. adding activated carbon to second mixture and filtering it to obtain decolorized second mixture;
g. inducing crystallization in the decolorized second mixture by temperature gradient treatment;
h. filtering the decolorized second mixture to obtain a second precipitate and a second filtrate;
i. suspending the second precipitate in a third aqueous alcoholic solution to obtain a third mixture;
j. filtering the third mixture to obtain a third precipitate and a third filtrate; and
k. drying the third precipitate to yield purified Rubusoside.
10 . The process of claim 9 wherein the first aqueous alcoholic solution in step
(b) is an methanol-water solution, with 75-99% methanol.
11 . The process of claim 9 wherein the second aqueous alcoholic solution in
step (e) is a methanol-water solution with 70-90% methanol.
12 . The process of claim 9 wherein the third aqueous alcoholic solution in step
(i) is an methanol-water solution with 90-99% methanol.
13 . The process of claim 9 wherein steps (c) and (g) inducing crystallization
comprises increasing the mixture temperature to 65-70° C. then gradually cooling it to 10° C. at a rate of 10° C. per hour with continuous mild agitation.
14 . The process of claim 9 wherein the aqueous alcoholic solution comprises
one or more organic solvents selected from the group consisting of methanol, ethanol, 1-propanol, and isopropanol.
15 . The process of claim 1 , wherein the purified Rubusoside has a purity greater than 95% on a dry basis.
16 . The process of claim 1 , wherein the purified Rubusoside has a purity greater than 98% on a dry basis.
17 . A product comprising high purity rubusoside of claim 1 , wherein the product is selected from the group consisting of food, beverage, pharmaceutical composition, tobacco, nutraceutical, oral hygienic composition, or cosmetic.
18 . A sweetener composition comprising high purity rubusoside of claim 1 .
19 . The sweetener composition of claim 18 , further comprising Rebaudioside A, enzymatically modified stevia, Rebaudioside D, a mixture of steviol glycosides with more than 95% (on dry basis) total steviol glycosides content, high intensity sweetener and natural flavor compound, caloric sweetener, or sucrose.
20 . The sweetener composition of claim 18 , further comprising one natural high intensity sweetener selected from the group consisting of: steviol glycosides including a purified sweet steviol glycoside mixture, stevioside, rebaudioside A, rebaudioside B, rebaudioside C, rebaudioside D, rebaudioside E, rebaudioside F, dulcoside A, dulcoside B, stevia, alpha-glucosyl stevia, fructosyl stevia, galactosyl stevia, beta-glucosyl stevia; siamenoside; mogroside IV; mogroside V; Luo Han Guo sweetener; monatin and its salts (monatin SS, RR, RS, SR); glycyrrhizic acid and its salts; curculin; thaumatin; monellin; mabinlin; brazzein; hernandulcin; phyllodulcin; glycyphyllin; phloridzin; trilobatin; baiyunoside; osladin; polypodoside A; pterocaryoside A; pterocaryoside B; mukurozioside; phlomisoside I; periandrin I; abrusoside A; cyclocarioside I; and combinations thereof.