Biomarker for determining mitochondrial damage in friedreich's ataxia
Compositions and methods for screening for a disease or a disorder associated with a deficiency in frataxin in a subject using biomarkers for diseases or disorders associated with a deficiency in frataxin are disclosed. The compositions and methods include determining the acetylation status of mitochondrial proteins. Also disclosed are methods of detecting progression of a disease or a disorder associated with a deficiency in frataxin in a subject and methods of monitoring effectiveness of a therapy for diseases or disorders associated with a deficiency in frataxin.
1. A kit for measuring levels of mitochondrial protein acetylation comprising an anti-acetyl-lysine-specific antibody and an antibody that specifically binds to a mitochondrial protein wherein the mitochondrial protein is selected from the group consisting of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 (NDUFA9), acetyl-CoA synthetase 2 (AceCS2), frataxin, complex II 30 kDa subunit, complex HI Rieske protein, NAD-dependent deacetylase sirtuin-3 (SIRT3), voltage-dependent anion-selective channel (VDAC), and combinations thereof.
2. The kit of claim 1 further comprising at least one of a reagent for processing a sample, a reagent for isolating mitochondria, and a reagent for measuring mitochondrial protein acetylation.