Aminomethylene pyrazolones with therapeutic activity

1. A method for the treatment of carcinoma, the method comprising:

administering to a subject suffering from carcinoma a compound having the structure according to formula III:

wherein:

X is NH or S;

R 1 is H or (1C-4C)alkyl;

R 2 is (1C-4C)alkyl, phenyl, or a monocyclic aromatic ring having one or more N—, O— or S— atoms in the ring, wherein the (1C-4C)alkyl, phenyl, or monocyclic aromatic ring is unsubstituted or substituted;

R 3 and R 4 are each independently H, (1C-6C)alkyl, (2C-6C) alkenyl, (2C-6C)alkynyl, cyano, a monocyclic aromatic ring having one or more N—, O— or S— atoms in the ring, a monocyclic non-aromatic ring having one or more N—, O— or S— atoms in the ring, wherein each is unsubsituted or substituted;

or R 3 and R 4 form together a pyrrolyl, imidazolyl, pyrazolyl, pyrrolidinyl, pyrrolinylimidazolidinyl, imidazolinyl, piperidyl, or piperazinylmorpholinyl, wherein each is unsubsituted or substituted;

or wherein R 3 and R 4 each independently represent hydrogen, methyl, ethyl or propyl or a group selected from list of structures consisting of:

or R 3 and R 4 form together a substituted ring as represented in the following structures:

R 5 is H, Cl, F, Br, Me, NO 2 , t-butyl, OCF 3 , OCH 3 , or CF 3 ;

R 6 is H, (1C-4C)alkyl, (1C-4C)alkyloxy, halo(1C-4C)alkyl, halo(1C-4C)alkyloxy, nitro or halogen;

R 7 is H, F, Cl, Br, Me, NO 2 , t-butyl, OCF 3 , OCH 3 , or CF 3 ;

or a pharmaceutically acceptable addition salt thereof.

2. The method according to claim 1 , wherein:

R 5 and R 6 are hydrogen;

R 7 is H.

3. The method according to claim 1 , wherein X is S.

4. The method according to claim 1 , wherein R 1 is H or (1C-4C)alkyl and R 2 is (1C-4C)alkyl or phenyl.

5. The method according to claim 1 , wherein R 3 is hydrogen.

6. The method according to claim 1 , wherein R 3 and R 4 represent both methyl or R 3 is hydrogen.

7. The method according to claim 1 , wherein the compound according to formula III is a compound having a structure according to formula II:

or a pharmaceutically acceptable addition salt thereof.

8. A method for the treatment of carcinoma, the method comprising:

administering to a subject suffering from carcinoma a compound having the structure according to formula I:

wherein:

X is NH or S;

R 1 is H or (1C-4C)alkyl;

R 2 is —Z or —Y—Z, wherein Y is —CH 2 — or —CH 2 —CH 2 —, and Z is thien-2-yl, N—methylpyrol-3-yl, benzo[b]thien-2-yl, 2-naphthalenyl, phenyl, or a monocyclic aromatic ring having one or more N—, O— or S— atoms in the ring, and wherein Z is unsubstituted or substituted;

R 3 and R 4 are each independently H, (1 C-6C)alkyl, (2C-6C) alkenyl, (2C-6C)alkynyl, cyano, (3C-6C)cycloalkyl, a monocyclic aromatic ring having one or more N—, O— or S— atoms in the ring, a monocyclic non-aromatic ring having one or more N—, O— or S— atoms in the ring, wherein each is unsubsituted or substituted;

or R 3 and R 4 form together a pyrrolyl, imidazolyl, pyrazolyl, pyrrolidinyl, pyrrolinylimidazolidinyl, imidazolinyl, piperidyl, piperazinylmorpholinyl, wherein each is unsubsituted or substituted;

R 5 is H or CF 3 ;

R 6 is H, (1C-4C)alkyl, (1C-4C)alkyloxy, halo(1C-4C)alkyl, halo(1C-4C)alkyloxy, nitro or halogen;

or a pharmaceutically acceptable addition salt thereof.

9. The method according to claim 1 , wherein the compound according to formula III is a compound according to formula I:

wherein:

R 2 is a substituted or unsubstituted monocyclic aromatic ring having one or more N—, O— or S— atoms in the ring;

R 5 is H or CF 3 ;

or a pharmaceutically acceptable addition salt thereof.

10. The method according to claim 8 , wherein:

X is S;

R 1 is H;

R 2 is Z and Z is substituted or unsubstituted phenyl;

or Z is substituted or unsubstituted thien-2-yl;

or Z is N-methylpyrol-3-yl or benzo[b]thien-2-yl or 2-naphthalenyl;

R 3 ,R 4 are H,H or H,CH 3 or CH 3 ,CH 3 ;

R 5 is H;

R 6 is H, halogen or methoxy;

or a pharmaceutically acceptable addition salt thereof.

11. The method according to claim 8 , wherein R 6 is methoxy.

12. A method for the treatment of carcinoma, the method comprising:

administering to a subject suffering from carcinoma a compound having the structure according to formula III:

wherein:

X is S;

R l is H, or CH 3 ;

R 2 is CF 3 , CH 3 , phenylethyl,

 wherein R a is H, F, Cl, Br, I, NO 2 , methyl, ethyl, isopropyl, t-butyl, methoxy or CF 3 and R b is H, Cl or CH 3 ;

R 3 , R 4 is H, H or H, CH 3 , or CH 3 , CH 3 or one of R 3 or R 4 is —CN or p-methoxyphenylmethyl or R 3 and R 4 together represent a ring

 on the nitrogen of Formula III to represent a piperidyl or R 3 and R 4 together represent a ring

 on the nitrogen of Formula III to pyrrolidinyl, or R 3 is methyl and R 4 is dichlorbenzyl

R 5 is H, Cl, F, Br, Me, NO 2 , t-butyl, OCF 3 , OCH 3 , or CF 3 ,

R 6 is H, F, Cl, Br, NO 2 , CH 3 , t-butyl, OCH 3 , OCF 3 , or CF 3 ;

R7 is H, F, Cl, Br, Me, NO2, t-butyl, OCF3, OCH3, or CF3,

or a pharmaceutically acceptable addition salt thereof.

13. The method according to claim 12 , wherein:

R 2 is CF 3 , CH 3 , phenylethyl,

 wherein R a is H, F, Cl, Br, I, NO 2 , methyl, ethyl, isopropyl, t-butyl, methoxy or CF 3 and R b is H, Cl or CH 3 ;

R 3 , R 4 is H, H or H, CH 3 , or CH 3 , CH 3 or one of R 3 or R 4 is —CN or p-methoxyphenylmethyl or R 3 and R 4 together represent a ring

 on the nitrogen of Formula III to represent a piperidyl or R 3 and R 4 together represent a ring

 on the nitrogen of Formula III to pyrrolidinyl, or R 3 is methyl and R 4 is dichlorbenzyl

R 5 is H, or Cl;

R 6 is H, F, Cl, NO 2 , CH 3 , t-butyl, OCH 3 or OCF 3 ;

R 7 is H, or Cl;

or a pharmaceutically acceptable addition salt thereof.

14. The method according to claim 12 , wherein:

R 1 is H;

R 2 is CF 3 , CH 3 , phenylethyl,

 wherein R a is H, F, Cl, Br, I, NO 2 , methyl, ethyl, isopropyl, t-butyl, methoxy or CF 3 and R ID is H, Cl or CH 3 ;

R 3 , R 4 is H, H or H, CH 3 , or together represent a ring

 on the nitrogen of Formula III to represent piperidyl, or R 3 is methyl and R 4 is dichlorbenzyl

R 5 is H;

R 6 is H, Cl, NO 2 , CH 3 , OCH 3 , OCF 3 ;

R 7 is H.

15. The method according to claim 12 , wherein:

R 1 H;

R 2 is CF 3 , CH 3 ,

 wherein R a is H, F, Cl, Br, I, methyl, ethyl, isopropyl, t-butyl or CF 3 and R b is H, Cl or CH 3 ;

R 3 , R 4 is H, H or H, CH 3 , or together represent a ring

 on the nitrogen of Formula III to represent piperidyl, or R 3 is methyl and R 4 is dichlorbenzyl

R 5 is H;

R 6 is H, Cl, NO 2 , CH 3 , OCH 3 , OCF 3 ;

R 7 is H;

or a pharmaceutically acceptable addition salt thereof.

16. The method according to claim 1 , wherein the carcinoma is selected from the group consisting of gastric cancer, bladder cancer, esophageal cancer, breast cancer, prostate cancer and pancreas cancer.

17. The method according to claim 16 wherein the carcinoma is prostate cancer.

18. The method according to claim 1 , wherein the treatment results in delaying, preventing, treating or reversing metastasis.

19. The method according to claim 1 , wherein the alkyl, phenyl or aromatic ring of R 2 is substituted with one or more groups selected from the group consisting of (1C-4C)alkyl, (1C-4C)alkyloxy, halo(1C-4C)alkyl, halo(1C-4C)alkyloxy, phenyloxy, phenylthio, halogen, and nitro.

20. The method according to claim 1 , wherein R 3 is a (1C-6C)alkyl, (2C-6C) alkenyl, (2C-6C)alkynyl, cyano, (3C-6C)cycloalkyl, phenyl, a monocyclic aromatic ring having one or more N—, O— or S— atoms in the ring, a monocyclic non-aromatic ring having one or more N—, O— or S— atoms in the ring and wherein R 3 is substituted with a hydroxyl, (1C-4C)alkoxy, phenyl, cycloalkyl, piperidyl, piperazinyl, furyl, thienyl, pirazinyl, pyrrolyl, 2H-pyrrolyl, pyrazolyl, isoxazolyl, isothiazolyl, pyrrolidonyl, pyrrolinyl, imidazolinyl, imidazolyl, or monocyclic aromatic ring having one or more N—, O— or S— atoms in the ring, wherein each substitution is unsubstituted or further substituted.

21. The method according to claim 20 , wherein the substitution hydroxyl, (1C-4C)alkoxy, phenyl, cycloalkyl, piperidyl, piperazinyl, furyl, thienyl, pirazinyl, pyrrolyl, 2H-pyrrolyl, pyrazolyl, isoxazolyl, isothiazolyl, pyrrolidonyl, pyrrolinyl, imidazolinyl, imidazolyl, or monocyclic aromatic ring having one or more N—, O— or S— atoms in the ring is further substituted with (1C-4C)alkyl, (1C-4C)alkyloxy, halo(1C-4C)alkyl, halo(1C-4C)alkyloxy, halogen, nitro or (1C-2C)dioxol forming a ring.

22. The method according to claim 1 , wherein R 4 is a (1C-6C)alkyl, (2C-6C) alkenyl, (2C-6C)alkynyl, cyano, (3C-6C)cycloalkyl, phenyl, a monocyclic aromatic ring having one or more N—, O— or S— atoms in the ring, a monocyclic non-aromatic ring having one or more N—, O— or S— atoms in the ring and wherein R 4 is substituted with a hydroxyl, (1C-4C)alkoxy, phenyl, cycloalkyl, piperidyl, piperazinyl, furyl, thienyl, pirazinyl, pyrrolyl, 2H-pyrrolyl, pyrazolyl, isoxazolyl, isothiazolyl, pyrrolidonyl, pyrrolinyl, imidazolinyl, imidazolyl, or monocyclic aromatic ring having one or more N—, O— or S— atoms in the ring, wherein each substitution is unsubstituted or further substituted.

23. The method according to claim 22 , wherein the substitution hydroxyl, (1C-4C)alkoxy, phenyl, cycloalkyl, piperidyl, piperazinyl, furyl, thienyl, pirazinyl, pyrrolyl, 2H-pyrrolyl, pyrazolyl, isoxazolyl, isothiazolyl, pyrrolidonyl, pyrrolinyl, imidazolinyl, imidazolyl, or monocyclic aromatic ring having one or more N—, O— or S— atoms in the ring is further substituted with (1C-4C)alkyl, (1C-4C)alkyloxy, halo(1C-4C)alkyl, halo(1C-4C)alkyloxy, halogen, nitro or (1C-2C)dioxol forming a ring.

24. The method according to claim 1 , wherein R 3 and R 4 form together a pyrrolyl, imidazolyl, pyrazolyl, pyrrolidinyl, pyrrolinylimidazolidinyl, imidazolinyl, piperidyl, or piperazinylmorpholinyl, wherein each of the foregoing is substituted with (1C-6C)alkyl, phenyl(1C-4C)alkyl, or phenylketo(1C-4C)alkyl.

25. The method according to claim 2 , wherein alkyl, phenyl or aromatic ring of R 2 is substituted with one or more groups selected from the group consisting of a (1C-4C)alkyl and a halogen.

26. The method according to claim 8 , wherein Z is phenyl or a monocyclic aromatic ring having one or more N—, O— or S— atoms in the ring, and wherein Z is substituted with one or more groups selected from the group consisting of (1 C-4C)alkyl, (1 C-4C)alkyloxy, halo(1C-4C)alkyl, halo(1C-4C)alkyloxy, phenyloxy, phenylthio, halogen, or nitro from (1C-4C)alkyl, (1C-4C)alkyloxy, halo(1C-4C)alkyl, halo(1C-4C)alkyloxy, phenyloxy, phenylthio, halogen, and nitro.

27. The method according to claim 8 , wherein Z is thien-2-yl substituted at position 3, 4 or 5 with a halogen.

28. The method according to claim 8 , wherein R 3 is (1C-6C)alkyl, (2C-6C) alkenyl, (2C-6C)alkynyl, cyano, (3C-6C)cycloalkyl, a monocyclic aromatic ring having one or more N—, O— or S— atoms in the ring, a monocyclic non-aromatic ring having one or more N—, O— or S— atoms in the ring and wherein R 3 is substituted with a hydroxyl, (1C-4C)alkoxy, cycloalkyl, piperidyl, piperazinyl, furyl, thienyl, pirazinyl, pyrrolyl, 2H-pyrrolyl, pyrazolyl, isoxazolyl, isothiazolyl, pyrrolidonyl, pyrrolinyl, imidazolinyl, imidazolyl, or a monocyclic aromatic ring having one or more N—, O— or S— atoms in the ring, wherein each substitution is unsubstituted or further substituted.

29. The method according to claim 28 , wherein the substitution hydroxyl, (1C-4C)alkoxy, cycloalkyl, piperidyl, piperazinyl, furyl, thienyl, pirazinyl, pyrrolyl, 2H-pyrrolyl, pyrazolyl, isoxazolyl, isothiazolyl, pyrrolidonyl, pyrrolinyl, imidazolinyl, imidazolyl, or a monocyclic aromatic ring having one or more N—, O— or S— atoms in the ring is further substituted with (1C-4C)alkyl, (1C-4C)alkyloxy, halo(1C-4C)alkyl, halo(1C-4C)alkyloxy, halogen, nitro or (1C-2C)dioxol forming a ring.

30. The method according to claim 8 , wherein R 4 is (1C-6C)alkyl, (2C-6C) alkenyl, (2C-6C)alkynyl, cyano, (3C-6C)cycloalkyl, a monocyclic aromatic ring having one or more N—, O— or S— atoms in the ring, a monocyclic non-aromatic ring having one or more N—, O— or S— atoms in the ring and wherein R 4 is substituted with a hydroxyl, (1C-4C)alkoxy, cycloalkyl, piperidyl, piperazinyl, furyl, thienyl, pirazinyl, pyrrolyl, 2H-pyrrolyl, pyrazolyl, isoxazolyl, isothiazolyl, pyrrolidonyl, pyrrolinyl, imidazolinyl, imidazolyl, or a monocyclic aromatic ring having one or more N—, O— or S— atoms in the ring, wherein each substitution is unsubstituted or further substituted.

31. The method according to claim 30 , wherein the substitution hydroxyl, (1C-4C)alkoxy, cycloalkyl, piperidyl, piperazinyl, furyl, thienyl, pirazinyl, pyrrolyl, 2H-pyrrolyl, pyrazolyl, isoxazolyl, isothiazolyl, pyrrolidonyl, pyrrolinyl, imidazolinyl, imidazolyl, a monocyclic aromatic ring having one or more N—, O— or S— atoms in the ring is further substituted with (1 C-4C)alkyl, (1 C-4C)alkyloxy, halo(1C-4C)alkyl, halo(1C-4C)alkyloxy, halogen, nitro or (1C-2C)dioxol forming a ring.

32. The method according to claim 8 , wherein R 3 and R 4 form together a pyrrolyl, imidazolyl, pyrazolyl, pyrrolidinyl, pyrrolinylimidazolidinyl, imidazolinyl, piperidyl, or piperazinylmorpholinyl, wherein each of the foregoing is substituted with (1C-6C)alkyl, phenyl(1C-4C)alkyl, or phenylketo(1C-4C)alkyl.

33. The method according to claim 9 , wherein the aromatic ring of R 2 is substituted with one or more groups selected from the group consisting of (1 C-4C)alkyl, (1C-4C)alkyloxy, halo(1C-4C)alkyl, halo(1C-4C)alkyloxy, phenyloxy, phenylthio, halogen, and nitro.

34. The method according to claim 9 , wherein R 3 is a (1C-6C)alkyl, (2C-6C) alkenyl, (2C-6C)alkynyl, cyano, (3C-6C)cycloalkyl, a monocyclic non-aromatic ring having one or more N—, O— or S— atoms in the ring and wherein R 3 is substituted with a hydroxyl, (1C-4C)alkoxy, cycloalkyl, piperidyl, piperazinyl, furyl, thienyl, pyrrolyl, 2H-pyrrolyl, pyrazolyl, isoxazolyl, isothiazolyl, pyrrolidonyl, pyrrolinyl, imidazolinyl, or imidazolyl, wherein each substitution is unsubstituted or further substituted.

35. The method according to claim 34 , wherein the substitution hydroxyl, (1C-4C)alkoxy, cycloalkyl, piperidyl, piperazinyl, furyl, thienyl, pyrrolyl, 2H-pyrrolyl, pyrazolyl, isoxazolyl, isothiazolyl, pyrrolidonyl, pyrrolinyl, imidazolinyl, imidazolyl is further substituted with (1C-4C)alkyl, (1C-4C)alkyloxy, halo(1C-4C)alkyl, halo(1C-4C)alkyloxy, halogen, nitro or (1C-2C)dioxol forming a ring.

36. The method according to claim 9 , wherein R 4 is a (1C-6C)alkyl, (2C-6C) alkenyl, (2C-6C)alkynyl, cyano, (3C-6C)cycloalkyl, a monocyclic non-aromatic ring having one or more N—, O— or S— atoms in the ring and wherein R 4 is substituted with a hydroxyl, (1C-4C)alkoxy, cycloalkyl, piperidyl, piperazinyl, furyl, thienyl, pyrrolyl, 2H-pyrrolyl, pyrazolyl, isoxazolyl, isothiazolyl, pyrrolidonyl, pyrrolinyl, imidazolinyl, or imidazolyl wherein each substitution is unsubstituted or further substituted.

37. The method according to claim 36 , wherein the substitution hydroxyl, (1C-4C)alkoxy, cycloalkyl, piperidyl, piperazinyl, furyl, thienyl, pyrrolyl, 2H-pyrrolyl, pyrazolyl, isoxazolyl, isothiazolyl, pyrrolidonyl, pyrrolinyl, imidazolinyl, imidazolyl is further substituted with (1C-4C)alkyl, (1C-4C)alkyloxy, halo(1C-4C)alkyl, halo(1C-4C)alkyloxy, halogen, nitro or (1C-2C)dioxol forming a ring.

38. The method according to claim 9 , wherein R 3 and R 4 form together a pyrrolyl, imidazolyl, pyrazolyl, pyrrolidinyl, pyrrolinylimidazolidinyl, imidazolinyl, piperidyl, piperazinylmorpholinyl, wherein each of the foregoing is substituted with (1C-6C)alkyl, phenyl(1C-4C)alkyl, or phenylketo(1C-4C)alkyl.

39. The method according to claim 10 , wherein Z is phenyl and is substituted at meta or para position, or at both positions, with one or two substituents selected from the group consisting of —NO 2 , halogen, CF 3 , (1C-4C)alkyl and methoxy.

40. The method according to claim 10 , wherein Z is thien-2-yl, and is substituted at least one of positions 3, 4 and 5 with halogen.

41. A method for treating a subject having a carcinoma, the method comprising:

administering to the subject a compound having the structure according to formula IV:

or a pharmaceutically acceptable addition salt thereof.

42. A method for treating a subject having a carcinoma, the method comprising:

administering to the subject a compound having the structure according to formula V:

or a pharmaceutically acceptable addition salt thereof.