Methods for modulating hair growth using truncated laminin-511
Disclosed are methods for the use of a truncated, recombinant laminin-511 for modifying hair growth as well as delivery devices, kits and methods for topically administering truncated, recombinant laminin-511. Furthermore disclosed are delivery devices, kits and methods using modulators of full-length laminin-511 expression or function to decrease hair growth in areas of unwanted hair growth.
1. A biodegradable or biocompatible microneedle device for application of a laminin-511 peptide to a subject, the device comprising an array of hollow microneedles comprising a composition comprising a truncated, recombinant laminin-511 peptide trimer and a pharmaceutically acceptable carrier in a therapeutically effective amount to increase scalp hair growth and to decrease scalp hair loss in a subject.
2. The microneedle device of claim 1 , wherein the laminin-511 peptide is a truncated, recombinant laminin-511 peptide trimer comprising an alpha-5 chain comprising a sequence identical to SEQ ID NO: 1; a beta-1 chain comprising a sequence identical to SEQ ID NO: 2; and a gamma-1 chain comprising a sequence identical to SEQ ID NO: 3, and conservative variants thereof.
3. The microneedle device of claim 1 , wherein the laminin-511 peptide is a truncated, recombinant laminin-511 peptide trimer comprising an alpha-5 chain comprising a sequence identical to SEQ ID NO: 4; a beta-1 chain comprising a sequence identical to SEQ ID NO: 2; and a gamma-1 chain comprising a sequence identical to SEQ ID NO: 3, and conservative variants thereof.
4. The microneedle device of claim 1 , wherein the laminin-511 peptide is a truncated, recombinant laminin-511 peptide trimer comprising an alpha-5 chain comprising a sequence identical to SEQ ID NO: 5; a beta-1 chain comprising a sequence identical to SEQ ID NO: 2; and a gamma-1 chain comprising a sequence identical to SEQ ID NO: 3, and conservative variants thereof.
5. The microneedle device of claim 1 , wherein said composition further comprises at least one secondary treatment product.
6. A method for delivering a laminin-511 peptide to dermal layers of a subject's scalp, the method comprising providing a biocompatible or biodegradable device comprising an array of hollow microneedles comprising a composition comprising a laminin-511 peptide and a pharmaceutically acceptable carrier in a therapeutically effective amount to a subject in need thereof to increase scalp hair growth and to decrease scalp hair loss in said subject; whereby said array is suited to be inserted into said subject's scalp with a pressure sufficient to deliver said composition to the dermal layers of said subject's scalp.
7. The method of claim 6 , wherein the laminin-511 peptide is a truncated, recombinant laminin-511 peptide trimer comprising an alpha-5 chain comprising a sequence identical to SEQ ID NO: 1; a beta -1 chain comprising a sequence identical to SEQ ID NO: 2; and a gamma-1 chain comprising a sequence identical to SEQ ID NO: 3,and conservative variants thereof.
8. The method of claim 6 , wherein the laminin-511 peptide is a truncated, recombinant laminin-511 peptide trimer comprising an alpha-5 chain comprising a sequence identical to SEQ ID NO: 4; a beta-1 chain comprising a sequence identical to SEQ ID NO: 2; and a gamma-1 chain comprising a sequence identical to SEQ ID NO: 3, and conservative variants thereof.
9. The method of claim 6 , wherein the laminin-511 peptide is a truncated, recombinant laminin-511 peptide trimer comprising an alpha-5 chain comprising a sequence identical to SEQ ID NO: 5; a beta-1 chain comprising a sequence identical to SEQ ID NO: 2; and a gamma-1 chain comprising a sequence identical to SEQ ID NO: 3, and conservative variants thereof.
10. The method of claim 6 , wherein the laminin-511 peptide is a full-length laminin-511 trimer comprising an alpha-5 chain comprising a sequence identical to SEQ ID NO:6; a beta-1 chain comprising a sequence identical to SEQ ID NO:7; and a gamma -1 chain comprising a sequence identical to SEQ ID NO:8, and conservative variants thereof.
11. The method of claim 6 , wherein said composition further comprises at least one secondary treatment product.
12. A method for increasing scalp hair growth and decreasing scalp hair loss in a subject, said method comprising providing a biocompatible or biodegradable-device comprising an array of hollow microneedles comprising a composition comprising a laminin-511 peptide and a pharmaceutically acceptable carder in a therapeutically effective amount to a subject in need thereof, whereby said device is inserted into said subject's scalp with a pressure sufficient to deliver said composition to the dermal layers of said subject's scalp.
13. The method of claim 12 , wherein the truncated, recombinant laminin-511 peptide comprises an alpha-5 chain comprising a sequence identical to SEQ ID NO: 1; a beta-1 chain comprising a sequence identical to SEQ ID NO: 2; and a gamma-1 chain comprising a sequence identical to SEQ ID NO: 3, and conservative variants thereof.
14. The method of claim 12 , wherein the truncated, recombinant laminin-511 peptide comprises an alpha-5 chain comprising a sequence identical to SEQ ID NO: 4; a beta-1 chain comprising a sequence identical to SEQ ID NO: 2; and a gamma-1 chain comprising a sequence identical to SEQ ID NO: 3, and conservative variants thereof.
15. The method of claim 12 , wherein the truncated, recombinant laminin-511 peptide comprises an alpha-5 chain comprising a sequence identical to SEQ ID NO: 5; a beta-1 chain comprising a sequence identical to SEQ ID NO: 2; and a gamma-1 chain comprising a sequence identical to SEQ ID NO: 3, and conservative variants thereof.
16. The method of claim 12 , wherein the composition comprises at least one secondary treatment product.
17. A kit for carrying out a procedure to increase scalp hair growth and to decrease scalp hair loss, the kit comprising one or more biodegradable or biocompatible microneedle device for application of a laminin-511 peptide to a subject, said device comprising an array of hollow microneedles comprising a composition comprising a truncated, recombinant laminin-511 peptide trimer and a pharmaceutically acceptable carrier in a therapeutically effective amount to increase scalp hair growth and to decrease scalp hair loss in a subject, and directions for use.
18. The kit of claim 17 , wherein the truncated, recombinant laminin-511 peptide comprises an alpha-5 chain comprising a sequence identical to SEQ ID NO: 1; a beta-1 chain comprising a identical to SEQ ID NO: 2; and a gamma-1 chain comprising a sequence identical to SEQ ID NO: 3, and conservative variants thereof.
19. The kit of claim 17 , wherein the truncated, recombinant laminin-511 peptide comprises an alpha-5 chain comprising a sequence identical to SEQ ID NO:4; a beta-1 chain comprising a sequence identical to SEQ ID NO:2; and a gamma-1 chain comprising a sequence identical to SEQ ID NO:3, and conservative variants thereof.
20. The kit of claim 17 , wherein the truncated, recombinant laminin-511 peptide comprises an alpha-5 chain comprising a sequence identical to SEQ ID NO:5; a beta-1 chain comprising a sequence identical to SEQ ID NO:2; and a gamma-chain comprising a sequence identical to SEQ ID NO:3, and conservative variants thereof.
21. The kit of claim 17 , wherein said one or more microneedle devises further comprising at least one secondary treatment product.