Medical image processor and storage medium
A medical image processor and storage medium are shown. According to one implementation, a medical image processor includes an input unit, an operation unit, a cell nucleus extracting unit, a fluorescent bright point extracting unit, a feature amount calculating unit, and an output unit. The input unit is used to input a cell shape image showing a shape of a cell and a fluorescent image showing expression of a specific protein as a fluorescent bright point. The operation unit is used to specify an analysis target region. The cell nucleus extracting unit extracts a region of a cell nucleus. The fluorescent bright point extracting unit extracts a fluorescent bright point. The feature amount calculating unit calculates a feature amount showing an expression amount of the specific protein. The output unit outputs the calculated feature amount.
1. A medical image processor comprising:
an input unit to input a cell shape image showing a shape of a cell in a tissue slice and a fluorescent image showing expression of a specific protein in the tissue slice as a fluorescent bright spot, the fluorescent image being obtained using a fluorescent substance included nanoparticle, which has an average particle diameter in the range including 30 nm to 800 nm;
an operation unit to specify an analysis target region from the cell shape image;
a cell nucleus extracting unit which extracts a region of a cell nucleus from the cell shape image;
a fluorescent bright point extracting unit which extracts a fluorescent bright point showing expression of the specific protein from the fluorescent image;
a feature amount calculating unit which calculates a feature amount showing an expression amount of the specific protein in the specified analysis target region based on the extracted cell nucleus region in the analysis target region specified with the operation unit and the fluorescent bright point; and
an output unit which outputs the calculated feature amount,
wherein the florescent substance included nanoparticle is a nanoparticle with a fluorescent substance dispersed inside, the feature amount calculating unit obtains information of a number of fluorescent bright points and a number of cell nuclei in the analysis target region specified with the operation unit, and based on the obtained number of cell nuclei and the number of fluorescent bright points, the feature amount calculating unit calculates the feature amount showing the expression amount of the specific protein in the specified analysis target region, and the feature amount calculating unit calculates a number of the fluorescent bright points in each cell nucleus in the specified analysis target region as the feature amount showing the expression amount of the specific protein, and
wherein the florescent bright point extracting unit extracts the fluorescent bright spot showing expression of the specific protein from the fluorescent image using an immunohistochemical (IHC) method.
2. The medical image processor of claim 1 ,
further comprising, a synthesis image unit which generates a synthesis image of the cell shape image and the fluorescent image,
wherein, the output unit outputs the synthesis image overlapped with an annotation showing a position of the analysis target region specified with the operation unit together with the feature amount.
3. The medical image processor of claim 1 , wherein,
the cell shape image and the fluorescent image are combined to form one synthetic image which shows the shape of the cell in the tissue slice and the expression of the specific protein in the tissue slice as the fluorescent bright point.
4. The medical image processor of claim 1 , wherein the fluorescent substance is a quantum dot.
5. A non-transitory computer-readable storage medium including a program for controlling a computer to function as:
an input unit to input a cell shape image showing a shape of a cell in a tissue slice and a fluorescent image showing expression of a specific protein in the tissue slice as a fluorescent bright point, the fluorescent image being obtained using a fluorescent substance included nanoparticle, which has an average particle diameter in the range including 30 nm to 800 nm;
an operation unit to specify an analysis target region from the cell shape image;
a cell nucleus extracting unit which extracts a region of a cell nucleus from the cell shape image;
a fluorescent bright point extracting unit which extracts a fluorescent bright point showing expression of the specific protein from the fluorescent image;
a feature amount calculating unit which calculates a feature amount showing an expression amount of the specific protein in the specified analysis target region based on the extracted cell nucleus region in the analysis target region specified with the operation unit and the fluorescent bright point; and
an output unit which outputs the calculated feature amount,
wherein the fluorescent substance included nanoparticle is a nanoparticle with a fluorescent substance dispersed inside, the feature amount calculating unit obtains information of a number of fluorescent bright points and a number of cell nuclei in the analysis target region specified with the operation unit, and based on the obtained number of cell nuclei and the number of fluorescent bright points, the feature amount calculating unit calculates the feature amount showing the expression amount of the specific protein in the specified analysis target region, and the feature amount calculating unit calculates a number of the fluorescent bright points in each cell nucleus in the specified analysis target region as the feature amount showing the expression amount of the specfic protein, and
wherein the fluorescent bright point extracting unit extracts the fluorescent bright spot showing expression of the specific protein from the fluorescent image using an immunohistochemical (IHC) method.
6. The non-transitory computer-readable storage medium of claim 5 , wherein the fluorescent substance is a quantum dot.