Acoustically responsive particles
Acoustically responsive particles and methods are provided for their use. Methods are provided for making and using tunable, monodisperse acoustically responsive particles and negative contrast acoustic particles, wherein the particles can contain a functional group available for covalent modification.
1. A method for synthesizing elastomeric negative contrast acoustic particles having a functional vinyl group available for covalent modification, the method comprising:
emulsifying an elastomer pre-polymer comprising the functional vinyl group with a catalyst in the presence of a surfactant under conditions sufficient to produce emulsion droplets;
curing the emulsion droplets under conditions sufficient to form stable elastomeric negative acoustic contrast particles that have the functional vinyl group available for covalent modification; and
contacting the negative acoustic contrast particles with biotin-PEG-tetrafluorophenyl azide under conditions sufficient to covalently modify the available vinyl functional group with biotin for binding to a target of interest.
2. The method of claim 1 , wherein the elastomer pre-polymer comprises a silicone material.
3. The method of claim 1 , wherein the elastomer pre-polymer comprises polyvinylmethylsiloxane (PVMS).
4. The method of claim 1 , wherein the surfactant is a nonionic triblock copolymer surfactant.
5. The method of claim 4 , wherein the nonionic triblock copolymer surfactant has greater than 50 end groups of polyethylene oxide (PEO).
6. The method of claim 5 , wherein the nonionic triblock copolymer surfactant has a hydrophile-lipophile (HLB) value greater than 24.
7. The method of claim 1 , wherein the target of interest comprises one of a cell, a protein, a receptor, an antibody, an antigen, a drug, virus, nucleic acid, a polysaccharide or a metabolite.