Immunological analysis method and reagent
Disclosed are an immunoassay method which can measure an antigen with high sensitivity and accuracy; and a reagent therefor. In the immunoassay method, an antigen-antibody reaction and/or a measurement is(are) carried out in the presence of a polycarboxylic acid type surfactant. The immunoassay reagent for use in the method is characterized by comprising the polycarboxylic acid type surfactant. By employing such a simple means that the polycarboxylic acid type surfactant is allowed to be present in the reaction and/or measurement system, non-specific reactions can be suppressed effectively even in a highly sensitive immunoassay, and an antigen can be measured accurately and specificity can be improved in the immunoassay.
1. An optical immunoagglutination measurement method for detecting an antigen in a sample, said method comprising:
carrying out an immunoagglutination antigen-antibody reaction in the presence of a polycarboxylic acid type surfactant, said polycarboxylic acid type surfactant being in a reaction system at a concentration of 0.001% to 3% by mass, said reaction system inclusive of the sample; and
measuring optically the immunoglutinnated sample with a portion of the reaction system to detect the presence of the antigen in the sample;
wherein said polycarboxylic acid type surfactant is a copolymer of (1) maleic acid and/or maleic anhydride and (2) diisobutylene; and/or a salt thereof, and wherein said immunoagglutination antigen-antibody reaction is a latex agglutination.
2. The method according to claim 1 , wherein the concentration of said polycarboxylic acid type surfactant in the reaction system is 0.005% to 1%.
3. The method according to claim 1 , wherein said polycarboxylic acid type surfactant is allowed to be present during from beginning of the antigen-antibody reaction to completion of the measurement.
4. The method according to claim 1 , wherein the polycarboxylic acid type surfactant is dissolved in a solution.
5. The method according to claim 1 , wherein the immunoagglutination antigen-antibody reaction and measurement is carried out on a sample from urine.
6. An optical immunoagglutination measurement method for detecting an antigen in a sample comprising:
carrying out an immunoagglutination antigen-antibody reaction in the presence of a polycarboxylic acid type surfactant, said polycarboxylic acid type surfactant being in a reaction system at an effective amount and said reaction system inclusive of the sample; and
measuring optically the immunoglutinnated sample with a portion of the reaction system to detect the presence of the antigen in the sample;
wherein said polycarboxylic acid type surfactant is a copolymer of (1) maleic acid and/or maleic anhydride and (2) diisobutylene; and/or a salt thereof, and wherein said immunoagglutination antigen-antibody reaction is a latex agglutination.
7. An immunoagglutination measurement method for detecting an antigen in a sample comprising:
carrying out an immunoagglutination antigen-antibody reaction in the presence of a polycarboxylic acid type surfactant, said polycarboxylic acid type surfactant being in a reaction system at an effective amount and said reaction system inclusive of the sample; and
measuring the immunoglutinnated sample with a portion of the reaction system to detect the presence of the antigen in the sample;
wherein said polycarboxylic acid type surfactant is a copolymer of (1) maleic acid and/or maleic anhydride and (2) diisobutylene; and/or a salt thereof, and wherein said immunoagglutination antigen-antibody reaction is a latex agglutination.
8. The method according to claim 1 , wherein the antigen is a protein marker, immunoglobulin, viral antigen, bacterial antigen, peptide hormone, bioactive amine, vitamin, prostaglandin, antibiotic, pesticide or environmental hormone.
9. The method according to claim 1 , wherein the test sample does not comprise LDL.