IP Library Granted Patent US 9,429,507
Granted Patent B2
US 9,429,507 · App. 14/390,276 · Granted Aug 30, 2016

Flow cell, analysis equipment and analysis method using same

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Quick Facts
Patent No.
US 9,429,507
App. No.
14/390,276
Granted
Aug 30, 2016
Kind
B2
Abstract

All of bio-related substances, such as cells or bacteria, are placed at single and independent positions. A flow cell according to the present invention is used for analyzing a bio-related substance and includes a flow passageway and an injection opening and a discharge opening that are connected to the flow passageway. The flow passageway is provided with trapping structural members for trapping the bio-related substance. The trapping structural members include a structure forming a dead water region in which the bio-related substance is trapped.

Claims (30)

1. A flow cell used for analyzing a bio-related substance, the flow cell comprising:

a flow passageway, and an injection opening and a discharge opening that are connected to the flow passageway,

wherein the flow passageway is provided with a trapping structural member that traps the bio-related substance, the trapping structural member including a structure forming a dead water region in which the bio-related substance is trapped,

wherein the trapping structural member includes a slit member having a slit that passes the bio-related substance, and an accommodating member disposed on a downstream side of the slit member and facing the slit member to accommodate the bio-related substance.

2. The flow cell according to claim 1 , wherein the accommodating member has a U-shaped curved shape.

3. The flow cell according to claim 2 , wherein the accommodating member includes a recess at a position facing the slit.

4. The flow cell according to claim 1 , wherein:

the trapping structural member is arranged in a matrix including a fluid of flow direction and a direction perpendicular to the fluid flow direction;

the trapping structural member is disposed at regular intervals in a row direction; and

a trapping structural member of an odd-numbered row is placed at substantially the center of adjacent trapping structural members placed at even-numbered rows.

5. The flow cell according to claim 1 , wherein that the trapping structural member has a height equal to a height of the flow passageway.

6. The flow cell according to claim 1 , wherein:

the trapping structural member includes a pair of parallel curved waveform structural members, the curved waveform shape having periodicity, the waveform of each period being symmetrical; and

one of the pair of structural members includes a slit on an upper end, and the other includes a slit on a lower end, each of the slits having a predetermined interval.

7. The flow cell according to claim 1 , wherein the trapping structural member has an increasingly higher trap rate toward the downstream side.

8. The flow cell according to claim 7 , wherein the trapping structural member has an increasingly greater size toward the downstream side.

9. The flow cell according to claim 7 , wherein the slit member has an increasingly greater slit interval toward the downstream side.

10. The flow cell according to claim 8 , wherein the trapping structural member has an increasingly greater number of rows toward the downstream side.

11. An analysis method for analyzing a bio-related substance using a flow cell including a flow passageway and an injection opening and a discharge opening that are connected to the flow passageway,

wherein the method comprises a step of forming a dead water region by a trapping structural member disposed in the flow passageway to trap the bio-related substance, wherein the trapping structural member includes a slit member having a slit that passes the bio-related substance, and an accommodating member disposed on a downstream side of the slit member and facing the slit member to accommodate the bio-related substance, and the slit has a large interval with respect to the bio-related substance, the interval being greater than the interval of a gap between the slit member and the accommodating member;

and a step of trapping the bio-related substance in the dead water region.

12. The analysis method according to claim 11 , wherein the bio-related substance is a bacterium, a cell, or a bound body of at least one of the bacterium and the cell and a fine particle.

13. The analysis method according to claim 11 , wherein the method comprises a step of detecting the trapped bio-related substance.

14. The analysis method according to claim 13 , wherein the step of detecting uses Raman, a bright field, a dark field, a phase difference, differential interference, fluorescence, light emission, or an electronic microscope.

15. The analysis method according to claim 13 , wherein the method comprises, after the step of detecting, a step of collecting the bio-related substance by varying a solution sending speed, or the solution sending speed and a solution sending direction.

16. An analysis device used for analyzing a bio-related substance, the analysis device comprising:

a flow cell including a flow passageway and an injection opening and a discharge opening that are connected to the flow passageway;

an injection mechanism that injects a sample via the injection opening; and

a detection mechanism that detects the bio-related substance trapped in the flow cell,

wherein the flow passageway is provided with a trapping structural member that traps the bio-related substance, wherein the trapping structural member includes a structure forming a dead water region in which the bio-related substance is trapped, and wherein the trapping structural member includes a slit member having a slit that passes the bio-related substance, and an accommodating member disposed on a downstream side of the slit member and facing the slit member to accommodate the bio-related substance.

Assignments (2)
CHANGE OF NAME AND ADDRESS Recorded Mar 30, 2020
From: HITACHI HIGH-TECHNOLOGIES CORPORATION
To: HITACHI HIGH-TECH CORPORATION
Reel/Frame 052259/0227 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 9, 2014
From: MATSUI, TAKUYA; MAESHIMA, MUNEO
To: HITACHI HIGH-TECHNOLOGIES CORPORATION
Reel/Frame 033919/0209 →