Composition comprising material for inhibiting SCF or receptor thereof for treating or preventing diseases associated with vascular permeability
The present invention relates to a composition comprising a material for inhibiting stem cell factor (SCF) or the receptor thereof for treating or preventing vascular permeability related diseases. Also, the present invention relates to a method for screening for an agent for treating vascular permeability related diseases, comprising the steps of: (a) treating a sample from a patient suspected of having a disease associated with vascular permeability with a candidate material; and (b) comparing the level of expression of the SCF or the receptor thereof with that of a control group. The present invention verifies SCF as a novel target for regulating vascular permeability. When the present invention is used, vascular permeability is decreased in diseases of the eye involving increased vascular permeability, thereby effectively treating or preventing vascular permeability related diseases. Also, vascular permeability can be effectively regulated using the composition. Further, the present invention can be provided as an effective means for screening an agent for treating vascular permeability related diseases by measuring the regulation of the expressions of SCF and c-Kit.
1. A method for treating or preventing vascular permeability related diseases comprising the steps of selecting a patient in need of treating or preventing vascular permeability related diseases, and administering a composition comprising a material for inhibiting SCF (stem cell factor) or the receptor thereof to the patient, wherein the vascular permeability related diseases is selected from the group consisting of
non-proliferative diabetic retinopathy,
ROP (retinopathy of prematurity),
age-related macular degeneration,
corneal dystrophy,
retinoschises,
Stargardt's disease,
autosomal dominant druzen,
Best's macular dystrophy,
cystoid macular edema,
ischemic retinopathy,
inflammation-induced retinal degenerative disease,
X-linked juvenile retinoschisis,
Malattia Leventinese (ML),
Doyne honeycomb retinal dystrophy, and
vascular endothelial cell related inflammatory disease.
2. The method for treating or preventing vascular permeability related diseases according to claim 1 , wherein the SCF receptor is C-Kit.
3. The method for treating or preventing vascular permeability related diseases according to claim 1 , wherein the material inhibiting SCF or the receptor thereof is selected from the group consisting of siRNA, an antisense oligonucleotide, an aptamer, and an antibody specific to the SCF or the receptor thereof.
4. The method for treating or preventing vascular permeability related diseases according to claim 3 , wherein the material inhibiting the SCF receptor is C-Kit siRNA or SCF receptor inhibiting antibody.
5. The method for treating or preventing vascular permeability related diseases according to claim 4 , wherein the C-Kit siRNA has the nucleotide sequence of SEQ ID NO:2, 3, 4, or 5.
6. The method for treating or preventing vascular permeability related diseases according to claim 1 , wherein the treatment of vascular permeability related diseases is performed by inhibiting the permeability of vascular endothelial cells.
7. A method for regulating vascular permeability comprising the steps of selecting a patient in need of regulating vascular permeability related diseases, and regulating the expression of SCF or the receptor thereof in vivo in the patient by using a composition comprising a material for inhibiting SCF or the receptor thereof, wherein the vascular permeability related diseases is selected from the group consisting of
non-proliferative diabetic retinopathy,
ROP (retinopathy of prematurity),
age-related macular degeneration,
corneal dystrophy,
retinoschises,
Stargardt's disease,
autosomal dominant druzen,
Best's macular dystrophy,
cystoid macular edema,
ischemic retinopathy,
inflammation-induced retinal degenerative disease,
X-linked juvenile retinoschisis,
Malattia Leventinese (ML),
Doyne honeycomb retinal dystrophy, and
vascular endothelial cell related inflammatory disease.
8. A method for screening an agent for treating vascular permeability related diseases comprising the following steps: (a) treating a sample from a patient suspected of having vascular permeability related diseases with a candidate material; (b) comparing the expression level of SCF or the receptor thereof with that of the untreated control group, and (c) identifying a candidate material showing decreased expression level of SCF or the receptor thereof as an agent for treating vascular permeability related diseases.
9. The method for screening an agent for treating vascular permeability related diseases according to claim 8 , wherein the SCF receptor is C-Kit.
10. The method for screening an agent for treating vascular permeability related diseases according to claim 9 , wherein the step (b) further comprises the step of comparing the phosphorylation of C-Kit with that of the control group.